PXD024457-1

PXD024457 is an original dataset announced via ProteomeXchange.

Title	Allosteric transcription stimulation by super elongation complex
Description	The super elongation complex (SEC) contains the positive transcription elongation factor b (P-TEFb) and a subcomplex, ELL2-EAF1, which stimulates transcription elongation by RNA polymerase II (Pol II). Here we report the cryo-EM structure of ELL2-EAF1 bound to a Pol II elongation complex at 2.8 Å resolution. The ELL2-EAF1 dimerization module directly binds the Pol II lobe, explaining how SEC delivers P-TEFb to Pol II. The same site on the lobe also binds the initiation factor TFIIF, consistent with SEC binding only after the transition from transcription initiation to elongation. Structure-guided functional analysis shows that elongation stimulation requires the dimerization module and an ELL2 protein linker that tethers this module to the Pol II protrusion. Our results show that SEC stimulates elongation allosterically and indicate that this stimulation involves stabilization of a further closed conformation of the Pol II active center cleft.
HostingRepository	PRIDE
AnnounceDate	2021-07-15
AnnouncementXML	Submission_2021-07-14_21:52:44.032.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Momchil Ninov
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-02 05:24:41	ID requested
⏵ 1	2021-07-14 21:52:44	announced

Dataset with its publication pending

submitter keyword: Transcription, RNA polymerase II, super elongation complex, ELL2, EAF1, P-TEFb, stimulation of transcription elongation, TFIIF, Pol II protrusion

Henning Urlaub
contact affiliation	Max-Planck-Institute for Biophysical Chemistry Bioanalytical Mass Spectrometry Group Am Fassberg 11 D-37077 Goettingen, Germany University Medical Center Goettingen Bioanalytics Institute for Clinical Chemistry Robert Koch Strasse 40 D-37075 Goettingen, Germany
contact email	henning.urlaub@mpibpc.mpg.de
lab head
Momchil Ninov
contact affiliation	Max Planck Institute for Biophysical Chemistry, Bioanalytical Mass Spectrometry - Göttingen, Germany University Medical Center, Institute for Clinical Chemistry, Bioanalytics Group - Göttingen, Germany
contact email	momchil.ninov@mpibpc.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024457
     1. Label: PRIDE project
     2. Name: Allosteric transcription stimulation by super elongation complex