PXD024442 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Regulation of the HTRA2 protease activity by PDZ domain-binding peptide ligands and the influence on HTRA2-specific protein interaction networks in retinal tissues |
Description | For the identification of HTRA2-specific protein interaction partners in retinal tissues of the house swine (Sus scrofa), we performed co-immunoprecipitation experiments with recombinant 6His-tag HTRA2 followed by quantitative LC-MS-based proteomics (see Fig. 2A and File S1). In brief, 2 µg recombinant 6His-tag HTRA2 (HTRA2 group) were spiked into 5 mg homogenized porcine retina and was subsequently enriched with the bounded protein interaction partners via Ni-NTA affinity purification (n=3). In addition, two further sample groups were treated with 10 µg UCF 101 (UCF-101 group) or 10 µg synthetic CDR peptide (CDR group) to unravel the influence of both molecules on the HTRA2-specific protein interactome in the retinal tissues (n=3 for each group). Eluate fractions of each group were separated by 1-D SDS PAGE (Fig. S1) and subsequently subjected to in-gel trypsin digestion. To distinguish unspecific from HTRA2 specific binders, we also included a control Ni-NTA bead group (CTRL group) for the quantitative MS analysis (n=3). |
HostingRepository | PRIDE |
AnnounceDate | 2021-10-11 |
AnnouncementXML | Submission_2021-10-10_23:29:50.441.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Carsten Schmelter |
SpeciesList | scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-03-01 06:18:25 | ID requested | |
⏵ 1 | 2021-10-10 23:29:51 | announced | |
Publication List
Schmelter C, Fomo KN, Perumal N, Pfeiffer N, Grus FH, Regulation of the HTRA2 Protease Activity by an Inhibitory Antibody-Derived Peptide Ligand and the Influence on HTRA2-Specific Protein Interaction Networks in Retinal Tissues. Biomedicines, 9(8):(2021) [pubmed] |
Keyword List
submitter keyword: HTRA2 |
Neuroprotection |
Retina |
Interaction partners |
Co-immunoprecipitation |
Mass spectrometry |
Contact List
Dr. Carsten Schmelter |
contact affiliation | Translational and Experimental Ophthalmology, University Medical Center in Mainz |
contact email | cschmelter@eye-research.org |
lab head | |
Carsten Schmelter |
contact affiliation | Universitätsmedizin Mainz |
contact email | cschmelter@eye-research.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024442
- Label: PRIDE project
- Name: Regulation of the HTRA2 protease activity by PDZ domain-binding peptide ligands and the influence on HTRA2-specific protein interaction networks in retinal tissues