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PXD024426-1

PXD024426 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExtracellular vimentin mimics VEGF and is a target for anti-angiogenic immunotherapy
DescriptionAnti-angiogenic anticancer therapies possess immune-stimulatory properties by counteracting pro-angiogenic molecular mechanisms. We report that tumor endothelial cells ubiquitously overexpress and secrete the intermediate filament protein vimentin through type III unconventional mechanisms. Extracellular vimentin is pro-angiogenic and functionally mimics VEGF action, while concomitantly acting as inhibitor of leukocyte-endothelial interactions. Targeting of extracellular vimentin presents a promising anti-angiogenic immunotherapy strategy against cancer. Here we describe profiling of the HUVEC (Human Umbilical Vein Endothelial Cell) proteome, with special emphasis on vimentin (variants) in the different fractions. We have evidence that vimentin is externalized and deposited in the matrix of cultured cells and is as such available for targeted antiangiogenic therapeutics. The aim of the experiment is to determine the protein variants of vimentin in the cell, the secretome, and in the matrix deposited by the cells.
HostingRepositoryPRIDE
AnnounceDate2022-03-29
AnnouncementXMLSubmission_2022-03-29_05:58:52.671.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSander Piersma
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-01 04:44:21ID requested
12022-03-29 05:58:53announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Cancer, angiogenesis, anti-angiogenic therapy, VEGF, vimentin, human
Contact List
Connie Jimenez
contact affiliationAmsterdam UMC, Vrije Universiteit Amsterdam, Medical Oncology, Cancer Center Amsterdam, OncoProteomics Laboratory, Amsterdam, Netherlands
contact emailc.jimenez@vumc.nl
lab head
Sander Piersma
contact affiliationAmsterdam UMC
contact emails.piersma@amsterdamumc.nl
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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