PXD024425-2

PXD024425 is an original dataset announced via ProteomeXchange.

Title	putative K29-linked substrates identification using sAB-K29
Description	We have identified K29-linked ubiquitination is involved in cell cycle regulation and enriched in midbody formation. To identify the substrates of K29-linked ubiquitination in the midbody, we soughtto perform immunoprecipitation (IP) under denaturing conditions to minimize the presence of noncovalently bound proteins. we synchronized HeLa cells to telophase to maximize the abundance of the midbody and lysed the cells using 8 M urea. The lysate was further diluted in 1 M urea, followed by IP using biotinylated sAB-K29. The enriched proteins were separated on an SDS-PAGE gel and subjected to label-free quantitative mass spectrometry analysis.
HostingRepository	PRIDE
AnnounceDate	2021-07-12
AnnouncementXML	Submission_2021-07-11_22:13:35.458.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qingyun Zheng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-01 02:37:57	ID requested
1	2021-07-11 04:07:13	announced
⏵ 2	2021-07-11 22:13:36	announced	2021-07-12: Updated project metadata.

Yu Y, Zheng Q, Erramilli SK, Pan M, Park S, Xie Y, Li J, Fei J, Kossiakoff AA, Liu L, Zhao M, K29-linked ubiquitin signaling regulates proteotoxic stress response and cell cycle. Nat Chem Biol, 17(8):896-905(2021) [pubmed]

10.1038/S41589-021-00823-5;

submitter keyword: K29-linked ubiquitination

Lei Liu
contact affiliation	Tsinghua university, Department of chemistry
contact email	lliu@tsinghua.edu.cn
lab head
Qingyun Zheng
contact affiliation	Department of Chemistry, Tsinghua University
contact email	zhengqytsinghua@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD024425

PRIDE project URI

• PRIDE
  1. PXD024425
     1. Label: PRIDE project
     2. Name: putative K29-linked substrates identification using sAB-K29