PXD024420-1

PXD024420 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic Consequences of the Deletion of Cytochrome P450 (CYP450) Reductase in Mice
Description	Microsomal cytochrome P450 (CYP450) reductase enzymes play a major role in drug and xenobiotic metabolism. Mice which are deficient in hepatic CYP450 reductase serve as excellent models in understanding CYP450 drug metabolism and alterations in the underlying biology. A reversed-phase nano-bore UPLC-MS-based proteomic analysis, using an untargeted data independent approach (DIA), has been utilized for liver tissue extracts to evaluate differences between the proteomes of wild type (C57Bl6) and hepatic P450 reductase mice (HRNTM). Statistically curated, differential expressed protein groups highlighted a variety of molecular and biological functions, including binding and catalytic related activities. Pathway enrichment analysis resulted in perturbation of lipid and energy related pathways, which included bile acid biosynthesis, fatty acid omega oxidation and tricarbonic acid (TCA) cycle as examples.
HostingRepository	PRIDE
AnnounceDate	2022-05-26
AnnouncementXML	Submission_2022-05-26_06:03:47.034.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lee Gethings
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-28 23:54:24	ID requested
⏵ 1	2022-05-26 06:03:47	announced
2	2023-11-14 08:52:23	announced	2023-11-14: Updated project metadata.
3	2024-10-22 05:37:00	announced	2024-10-22: Updated project metadata.

Publication List

10.1016/J.JCHROMB.2021.122803;

10.1016/J.JCHROMB.2021.122803;

Keyword List

submitter keyword: Proteomics
Cytochrome P450
lipid metabolism
bile acids
glutathione, TCA cycle.

submitter keyword: Proteomics

Cytochrome P450

lipid metabolism

bile acids

glutathione, TCA cycle.

Contact List

Lee Gethings
contact affiliation	Scientific Operations, Waters
contact email	lee_gethings@waters.com
lab head
Lee Gethings
contact affiliation	Waters Corporation
contact email	lee_gethings@waters.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD024420
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD024420

PRIDE project URI

Repository Record List

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