PXD024398-1

PXD024398 is an original dataset announced via ProteomeXchange.

Title	Proteomic landscape of SARS-CoV-2- and MERS-CoV-infected primary human renal epithelial cells
Description	Acute kidney injury is strongly associated with mortality in COVID-19 patients. However, host cell changes underlying infection of kidney cells with SARS-CoV-2 remain unknown and prevent understanding of the mechanisms leading to renal pathology. Here, we carried out extensive, dual-level proteomic analyses of primary human kidney cells infected with SARS-CoV-2 or MERS-CoV to disseminate virus and cell type specific changes. We established cellular infection models of proximal and distal tubular epithelial cells derived from human donors. Infected cells were analyzed by quantitative translatome and whole-cell proteomics over time. Our findings reveal shared pathways modified upon infection with both viruses, as well as SARS-CoV-2 specific host cell modulation driving key changes in innate immune activation, cellular protein quality control and mitochondrial biology. In addition, we identified extensive modulation in pathways associated with kidney failure that were instinctively regulated in a virus- and cell type-specific manner. Our findings provide an overview of the effects of SARS-CoV-2 or MERS-CoV infection on primary kidney cells revealing key pathways and highlighting potential treatment options.
HostingRepository	PRIDE
AnnounceDate	2022-01-30
AnnouncementXML	Submission_2022-01-30_14:00:04.003.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aneesha Kohli
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; 6x(13)C labeled residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-26 04:50:44	ID requested
⏵ 1	2022-01-30 14:00:04	announced

Dataset with its publication pending

submitter keyword: human, mePROD, TMD, TMT, coronavirus, SARS-CoV-2, MERS-CoV

Christian Münch
contact affiliation	Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt am Main, Germany Frankfurt Cancer Institute, Frankfurt am Main, Germany Cardio-Pulmonary Institute, Frankfurt am Main, Germany
contact email	ch.muench@em.uni-frankfurt.de
lab head
Aneesha Kohli
contact affiliation	Institute of Biochemistry II, Goethe University, Frankfurt am Main
contact email	aneeshak19@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD024398

PRIDE project URI

• PRIDE
  1. PXD024398
     1. Label: PRIDE project
     2. Name: Proteomic landscape of SARS-CoV-2- and MERS-CoV-infected primary human renal epithelial cells