PXD024381-1

PXD024381 is an original dataset announced via ProteomeXchange.

Title	Corynebacterium diphtheriae proteome adaptation to cell culture medium and serum
Description	ost-pathogen interactions are often studied in vitro using primary or immortal cell lines. This set-up avoids ethical problems of animal testing and has the additional advantage of lower costs. However, the influence of cell culture media on bacterial growth and metabolism is not considered or investigated in most cases. To address this question growth and proteome adaptation of Corynebacterium diphtheriae strain ISS3319 were investigated in this study. Bacteria were cultured in standard growth medium, cell culture medium and fetal calf serum. Mass spectrometric analyses and label-free protein quantification hint to an increased bacterial pathogenicity when grown in cell culture medium as well as an influence of the growth medium on the cell envelope.
HostingRepository	PRIDE
AnnounceDate	2021-03-12
AnnouncementXML	Submission_2021-03-12_00:00:52.858.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jens Möller
SpeciesList	scientific name: Corynebacterium diphtheriae bv. mitis str. ISS 3319; NCBI TaxID: 1450520;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-25 02:57:48	ID requested
⏵ 1	2021-03-12 00:00:53	announced

Dataset with its publication pending

submitter keyword: diphtheria, host-pathogen interaction, label-free, metabolic pathway, proteomics

Andreas Burkovski
contact affiliation	Department Biologie Professur für Mikrobiologie (Prof. Dr. Burkovski)
contact email	andreas.burkovski@fau.de
lab head
Jens Möller
contact affiliation	FAU Erlangen
contact email	Jens.moeller@fau.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD024381

PRIDE project URI

• PRIDE
  1. PXD024381
     1. Label: PRIDE project
     2. Name: Corynebacterium diphtheriae proteome adaptation to cell culture medium and serum