PXD024369-1

PXD024369 is an original dataset announced via ProteomeXchange.

Title	Mapping of immunopeptidome of cancer immunotherapy with oncolytic virus and checkpoint blockade
Description	The elucidation of therapy-induced changes to the class I major histocompatibility complex (MHC-I)-bound tumor antigens is crucial for understanding immune-mediated tumor eradication and identifying potential targets for peptide vaccines to enhance the efficacy of immunotherapies. Here, we investigated how oncolytic reovirus therapy with and without immune checkpoint blockade (ICB) alters the tumor peptide-MHC repertoire. Using mass spectrometry analysis of immunoaffinity purified MHC peptides, we first showed that changes to the MHC immunopeptidome following reovirus treatment is cancer type-dependent, where a murine fibrosarcoma model displayed quantitative and qualitative variance in differentially expressed peptides (DEPs) as compared to those identified in a murine ovarian cancer model. We then determined that the combination therapy of reovirus and ICB in the fibrosarcoma model resulted in higher numbers of DEPs relative to either monotherapy alone. Most importantly, we identified reovirus and ICB-induced MHC peptides that are biologically active in stimulating interferon-gamma response in cognate CD8+ T cells, which likely contribute to cancer immunoediting. These findings highlight the importance of therapy-induced changes to the MHC immunopeptidome in shaping the underlying anti-tumor immune responses during reovirus and ICB combination therapy.
HostingRepository	PRIDE
AnnounceDate	2021-12-06
AnnouncementXML	Submission_2021-12-05_17:03:52.587.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024369
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Youra Kim
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-24 10:35:09	ID requested
⏵ 1	2021-12-05 17:03:52	announced

Dataset with its publication pending

submitter keyword: Murine, fibrosarcoma, oncolytic virus, immune checkpoint blockade, antitumor immunity, MHC immunoprecipitation, LC-MS/MS, LC-SPS-MS3

Shashi Gujar
contact affiliation	Departments of Pathology, Microbiology & Immunology, and Biology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada
contact email	shashi.gujar@dal.ca
lab head
Youra Kim
contact affiliation	Dalhousie University
contact email	youra.kim@dal.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024369
     1. Label: PRIDE project
     2. Name: Mapping of immunopeptidome of cancer immunotherapy with oncolytic virus and checkpoint blockade