PXD024352-2

PXD024352 is an original dataset announced via ProteomeXchange.

Title	Deletion of the transcription factors Hsf1, Msn2 and Msn4 in yeast uncovers transcriptional reprogramming in response to proteotoxic stress
Description	The heat shock response is an ancient and ubiquitous program allowing organisms to survive adverse environmental conditions. In S. cerevisiae, three transcription factors, Hsf1, Msn2 and Msn4, are thought to regulate the stress response. While Msn2/4 can be deleted, Hsf1 is essential. By combining the depletion of Hsf1 with the deletion of Msn2 and Msn4, we were able to switch off the central stress response. We show that the transcription factors Hsf1 and Msn2/4 follow different strategies and regimes: Whereas Msn2/4 are responsible for a broad metabolic response, Hsf1 triggers a direct chaperone response to stabilize and repair unfolded proteins. Exposure of cells lacking Msn2/4 and Hsf1 to thermal stress resulted in massive protein aggregation. Comparison with wildtype yeast revealed that among the proteins rescued by the stress response are many essential proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:28:09.131.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Moritz Mühlhofer
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-24 04:09:02	ID requested
1	2024-01-31 09:31:42	announced
⏵ 2	2024-10-22 06:28:09	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Hsf1, Msn4, Msn2, Heat shock response

Johannes Buchner
contact affiliation	Technische Universität München Lichtenbergstraße 4 85748 Garching Deutschland
contact email	johannes.buchner@tum.de
lab head
Moritz Mühlhofer
contact affiliation	Dept. Chemie Lichtenbergstraße 4 85748 Garching
contact email	moritz.muehlhofer@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024352
     1. Label: PRIDE project
     2. Name: Deletion of the transcription factors Hsf1, Msn2 and Msn4 in yeast uncovers transcriptional reprogramming in response to proteotoxic stress