PXD024343-1

PXD024343 is an original dataset announced via ProteomeXchange.

Title	Coiled-Coil Domain-Containing 68 Downregulation Promotes Colorectal Cancer Cell Growth by Inhibiting ITCH-Mediated CDK4 Degradation
Description	Coiled-coil domain-containing 68 (CCDC68) plays different roles in cancer and is predicted as a tumor suppressor in human colorectal cancer (CRC). However, the specific role of CCDC68 in CRC and the underlying mechanisms remain unknown. Here, we showed that CCDC68 expression was lower in CRC than in corresponding normal tissues, and CCDC68 level was positively correlated with disease-free survival. Ectopic expression of CCDC68 decreased CRC cell proliferation in vitro and suppressed the growth of CRC xenograft tumors in vivo. CCDC68 caused G0/G1 cell cycle arrest, downregulated CDK4, and upregulated ITCH, the E3 ubiquitin ligase responsible for CDK4 protein degradation. This increased CDK4 degradation, which decreased CDK4 protein levels and inhibited CRC tumor growth. Collectively, the present results identify a novel CDK4 regulatory axis consisting of CCDC68 and ITCH, which suggest that CCDC68 is a promising target for the treatment of CRC.
HostingRepository	PRIDE
AnnounceDate	2021-04-14
AnnouncementXML	Submission_2021-04-14_05:10:34.101.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	wangcong wangcong
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-24 02:17:20	ID requested
⏵ 1	2021-04-14 05:10:34	announced

Dataset with its publication pending

submitter keyword: CCDC68, colorectal cancer, CDK4 degradation, ITCH, cell proliferation

Cong Wang
contact affiliation	Wenzhou Medical University (Zhejiang, China)
contact email	279859055@qq.com
lab head
wangcong wangcong
contact affiliation	Wenzhou Medical University
contact email	279859055@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024343
     1. Label: PRIDE project
     2. Name: Coiled-Coil Domain-Containing 68 Downregulation Promotes Colorectal Cancer Cell Growth by Inhibiting ITCH-Mediated CDK4 Degradation