PXD024336 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Newly translated protein misfolding after heat shock and translation during heat shock |
Description | Correct and efficient folding of nascent proteins to their native state requires support from the protein homeostasis network. We set to examine which newly translated proteins are less thermostable to infer which polypeptides require more time to fold. More specifically, we sought to determine which newly translated proteins are more susceptible to misfolding and aggregation under heat stress using pulse SILAC. These proteins were abundant, shorter, and highly ordered, with a potentially larger hydrophobic core as suggested by their higher hydrophobicity. Notably these proteins contain more β-sheets that typically require more time for folding and were enriched for Hsp70/Ssb and TRiC/CCT binding motifs, suggesting a higher demand for chaperone-assisted folding. These polypeptides were also more often components of stable protein complexes. All evidence combined suggests that a specific subset of newly translated proteins requires more time following synthesis to reach a thermostable native state in the cell. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:52:11.770.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mang Zhu |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | impact II |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-02-24 02:11:07 | ID requested | |
1 | 2022-08-11 16:26:25 | announced | |
⏵ 2 | 2023-11-14 08:52:12 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: proteomics,Protein folding, protein aggregation, proteostasis, heat stress |
Contact List
Thibault Mayor |
contact affiliation | Michael Smith Laboratories, The University of British Columbia |
contact email | mayor@msl.ubc.ca |
lab head | |
Mang Zhu |
contact affiliation | UBC |
contact email | mang.zhu@hotmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024336
- Label: PRIDE project
- Name: Newly translated protein misfolding after heat shock and translation during heat shock