PXD024335-1

PXD024335 is an original dataset announced via ProteomeXchange.

Title	Autophagosome content profiling - STAR Protocol Data Set
Description	The ascorbate peroxidase APEX2 is commonly used to study the neighborhood of a protein of interest by proximity-dependent biotinylation. Here, we describe a protocol for sample processing compatible with immunoblotting and mass spectrometry that is suitable to specifically map the content of autophagosomes and potentially other transient vesicles without the need of subcellular fractionation. By combining live-cell biotinylation with proteinase K digestion of cell homogenates, proteins enriched in membrane-protected compartments can be readily enriched and identified.
HostingRepository	PRIDE
AnnounceDate	2021-04-06
AnnouncementXML	Submission_2021-04-06_03:10:00.023.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Behrends
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-24 01:16:50	ID requested
⏵ 1	2021-04-06 03:10:00	announced

Zellner S, Schifferer M, Behrends C, Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling. Mol Cell, 81(6):1337-1354.e8(2021) [pubmed]

submitter keyword: Human, APEX2, proximity proteomics, proteinase K digest

Christian Behrends
contact affiliation	Munich Cluster for Systems Neurology (SyNergy), Ludwig-Maximilians-Universität München
contact email	christian.behrends@mail03.med.uni-muenchen.de
lab head
Christian Behrends
contact affiliation	SyNergy, LMU München
contact email	christian.behrends@mail03.med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD024335

PRIDE project URI

• PRIDE
  1. PXD024335
     1. Label: PRIDE project
     2. Name: Autophagosome content profiling - STAR Protocol Data Set