PXD024321-1

PXD024321 is an original dataset announced via ProteomeXchange.

Title	A SUPERFAMILY-WIDE ACTIVITY ATLAS OF SERINE HYDROLASES IN DROSOPHILA MELANOGASTER
Description	The serine hydrolase (SH) superfamily is perhaps, one of the largest functional enzyme classes in all forms of life, and consists of proteases/peptidases, lipases, and carboxylesterases as representative members. Consistent with the name of this superfamily, all members, without any exception to date, use a nucleophilic serine residue in the enzyme active site to perform hydrolytic-type reactions via a two-step ping-pong mechanism involving a covalent enzyme intermediate. Given the highly conserved catalytic mechanism, this superfamily has served as a classical prototype in the development of several platforms of the chemical proteomics technique, activity-based protein profiling (ABPP), to globally interrogate the functions of its different members in various native, yet complex, biological settings. While ABPP-based proteome-wide activity atlas’ for SH activities are available in numerous organisms, including humans, to the best of our knowledge, such an analysis for this superfamily is lacking in any insect model. To address this, here, we first report a bioinformatics analysis towards the identification and classification of non-redundant SHs in Drosophila melanogaster. Following up on this in silico analysis, leveraging discovery chemoproteomics, we identify and globally map the activities of the SH superfamily during various developmental stages and in different adult tissues of Drosophila. Finally, as proof of concept of the utility of this activity atlas, we highlight sexual dimorphism in SH activities across different tissues in adult Drosophila melanogaster, and together, we prospect new research directions, resources and tools that this study can provide to the fly community.
HostingRepository	PRIDE
AnnounceDate	2021-04-08
AnnouncementXML	Submission_2021-04-07_23:36:07.342.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Siddhesh Kamat
SpeciesList	scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-23 05:21:56	ID requested
⏵ 1	2021-04-07 23:36:07	announced

Dataset with its publication pending

submitter keyword: Chemoproteomics, ABPP, Drosophila, Serine Hydrolases

Siddhesh S. Kamat
contact affiliation	Associate Professor, Department of Biology, IISER Pune
contact email	siddhesh@iiserpune.ac.in
lab head
Siddhesh Kamat
contact affiliation	Indian Institute of Science Education and Research Pune
contact email	siddhesh@iiserpune.ac.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024321
     1. Label: PRIDE project
     2. Name: A SUPERFAMILY-WIDE ACTIVITY ATLAS OF SERINE HYDROLASES IN DROSOPHILA MELANOGASTER