PXD024316-1

PXD024316 is an original dataset announced via ProteomeXchange.

Title	ACONITASE 3 is post-translationally controlled in response to mitochondrial stress signals in Arabidopsis
Description	Mitochondria are tightly embedded within metabolic and regulatory networks that optimize plant performance upon environmental challenges. The best-known mitochondrial retrograde signaling pathway involves stress-induced activation of the transcription factor ANAC017, which mediates the onset of protective responses upon stress-induced mitochondrial dysfunction in Arabidopsis. Post-translational control of the elicited responses, in contrast, remains poorly understood. Previous studies linked protein phosphatase 2A subunit PP2A-B’γ, a key negative regulator of stress responses, with reversible phosphorylation of ACONITASE 3 (ACO3). Here we report on ACO3 and its phosphorylation at Ser91 as regulatory components induced by mitochondrial dysfunction. Targeted mass spectrometry-based proteomics revealed that the abundance and phosphorylation of ACO3 increased under stress, and that this required signaling through ANAC017. Phosphomimetic mutation at ACO3-Ser91 and the accumulation of ACO3S91D-YFP, in turn, promoted the expression of stress related genes and ACO3 function associated with plant tolerance against UV-B or antimycin A-induced mitochondrial dysfunction. These findings positioned ACO3 both as a target and modulator of mitochondrial dysfunction signaling, critical in the attainment of stress tolerance in Arabidopsis leaves.
HostingRepository	PRIDE
AnnounceDate	2021-06-08
AnnouncementXML	Submission_2021-06-07_21:44:25.654.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024316
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jesús Pascual
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-23 04:21:04	ID requested
⏵ 1	2021-06-07 21:44:26	announced

Dataset with its publication pending

submitter keyword: aconitase, mitochondrial dysfunction response, phosphorylation, post-translational regulation

Saijaliisa Kangasjärvi
contact affiliation	2Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, FI-00014 Helsinki, Finland Department of Agricultural Sciences, Faculty of Agriculture and Forestry, University of Helsinki, FI-00014 Helsinki, Finland
contact email	saijaliisa.kangasjarvi@helsinki.fi
lab head
Jesús Pascual
contact affiliation	University of Turku
contact email	jepavaz@utu.fi
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024316
     1. Label: PRIDE project
     2. Name: ACONITASE 3 is post-translationally controlled in response to mitochondrial stress signals in Arabidopsis