PXD024272-2
PXD024272 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | In-depth proteome analysis of Hela cell culture conditioned media containing fetal bovine serum. |
Description | We performed DIA-MS based proteomic analysis of Hela cells conditioned media containing fetal bovine serum by albumin (Alb)-depletion and non-depletion. In addition, we attempted to enrich secreted proteins by reducing the volume of culture medium (10mL, 5mL and 2mL). The supernatants of each culture condition were subjected to albumin depletion followed by DIA-MS. We further analyzed proteins in the conditioned media of HeLa cells with and without tumor necrosis factor (TNF) stimulation. |
HostingRepository | jPOST |
AnnounceDate | 2022-02-22 |
AnnouncementXML | Submission_2022-09-18_03:54:07.559.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | 中村 蓮 |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxymethyl-L-cysteine; unknown modification |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-02-21 20:51:11 | ID requested | |
1 | 2022-02-21 07:00:04 | announced | |
⏵ 2 | 2022-09-18 03:54:08 | announced | 2022-09-18: Updated FTP location. |
Publication List
Nakamura R, Nakajima D, Sato H, Endo Y, Ohara O, Kawashima Y, A Simple Method for In-Depth Proteome Analysis of Mammalian Cell Culture Conditioned Media Containing Fetal Bovine Serum. Int J Mol Sci, 22(5):(2021) [pubmed] |
Keyword List
submitter keyword: Proteome analysis, Secreted Proteins, Albumin removal, conditioned medium, Fetal bovine serum, DIA |
Contact List
Yusuke Kawashima | |
---|---|
lab head | |
中村 蓮 | |
contact affiliation | 公益財団法人かずさDNA研究所 |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001098/ |