PXD024245-1

PXD024245 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Coupling of Cdc20 inhibition and activation by BubR1
Description	Tight regulation of the APC/C-Cdc20 ubiquitin ligase that targets Cyclin B1 for degradation is important for mitotic fidelity. The spindle assembly checkpoint (SAC) inhibits Cdc20 through the mitotic checkpoint complex (MCC). In addition, phosphorylation of Cdc20 by Cyclin B1-Cdk1 independently inhibits APC/C-Cdc20 activation. This creates a conundrum for how Cdc20 gets activated prior to Cyclin B1 degradation. Here we show that the MCC component BubR1 harbours both Cdc20 inhibition and activation activities, allowing for cross-talk between the two Cdc20 inhibition pathways. Specifically BubR1 acts as a substrate specifier for PP2A-B56 to enable efficient Cdc20 dephosphorylation in the MCC. A mutant Cdc20 mimicking the dephosphorylated state escapes a mitotic checkpoint arrest arguing that restricting Cdc20 dephosphorylation to the MCC is important. Collectively our work reveals how Cdc20 can be dephosphorylated in the presence of Cyclin B1-Cdk1 activity without causing premature anaphase onset.
HostingRepository	MassIVE
AnnounceDate	2021-03-17
AnnouncementXML	Submission_2021-03-17_12:51:10.871.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mark Adamo
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Oxidation; Phospho; TMT6plex
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-18 14:24:24	ID requested
⏵ 1	2021-03-17 12:51:11	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Cdc20, BubR1, APC/C, ubiquitin ligase, Cyclin B1, mitosis, spindle, SAC, MCC, PP2A-B56, Cdk1, dephosphorylation, anaphase

submitter keyword: Cdc20, BubR1, APC/C, ubiquitin ligase, Cyclin B1, mitosis, spindle, SAC, MCC, PP2A-B56, Cdk1, dephosphorylation, anaphase

Contact List

Arminja Kettenbach
contact affiliation	The Geisel School of Medicine at Dartmouth
contact email	arminja.n.kettenbach@dartmouth.edu
lab head
Mark Adamo
contact affiliation	Dartmouth College
contact email	mark.e.adamo@dartmouth.edu
dataset submitter

Full Dataset Link List

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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086910/