PXD024244 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic differences between methicillin resistant Staphylococcus aureus showing synergistic effects with cannabinoids |
Description | Methicillin-resistant Staphylococcus aureus (MRSA) is a major human pathogen in both community and health care settings, which causes a wide range of infections. Its resistance to β-lactam antibiotics and methicillin in particular, greatly complicates treatment options and success rate due to the limited number of antibiotics with activity against MRSA. To further the development of alternative therapeutics, the mechanisms that mediate antibiotic resistance in MRSA need to be fully understood. Cannabinoid compounds including cannabidiol (CBD), tetrahydrocannabinol (THC) and cannabinol (CBN) have shown promise as potential antibiotic adjuvants. In the present study, MRSA cells were subjected to antibiotic stress from methicillin in combination with three cannabinoid compounds, and subsequently analysed using metaproteomics to assess the metabolic response. Subjecting MRSA to methicillin made the cells more viable and increased their energy production, as well as upregulation of penicillin-binding protein 2 (PBP2). The cannabinoids all showed antimicrobial activity against MRSA, and inhibited the energy production of the cells as well as PBP2 when used in combination with methicillin. Furthermore, all three cannabinoid compounds inhibited resistance mechanisms in MRSA, resulting in a decrease in the minimum inhibitory concentration (MIC) of methicillin when used in combination. |
HostingRepository | PRIDE |
AnnounceDate | 2022-01-18 |
AnnouncementXML | Submission_2022-01-18_10:04:58.657.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD024244 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Jan Struckmann Poulsen |
SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-02-18 10:12:11 | ID requested | |
⏵ 1 | 2022-01-18 10:04:59 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Antibiotic resistance, MRSA, Methicillin, Cannabinoids, Hormesis, Proteomics |
Contact List
Jeppe Lund Nielsen |
contact affiliation | Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, DK-9220, Aalborg East, Denmark |
contact email | jln@bio.aau.dk |
lab head | |
Jan Struckmann Poulsen |
contact affiliation | Aalborg University |
contact email | jsp@bio.aau.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD024244 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024244
- Label: PRIDE project
- Name: Proteomic differences between methicillin resistant Staphylococcus aureus showing synergistic effects with cannabinoids