PXD024098-2

PXD024098 is an original dataset announced via ProteomeXchange.

Title	Cryptococcus neoformans LC-MS/MS
Description	Cryptococcus neoformans is an invasive human fungal pathogen, which causes pneumonia and meningoencephalitis in both healthy and immunodeficient individuals, resulting 181,100 deaths each year. Studies have demonstrated that copper detoxification machineries play critical functions in modulating C. neoformans fitness and pathogenicity in the host lung tissue. Pulmonary C. neoformans infection provokes the generation of toxic copper bombardment, which in return activate detoxification process in fungal cells. However, the molecular mechanism on how Cu inhibits C. neoformans in proliferation remains unclear. Here, using Cu detoxification gene knockout strains we demonstrated that exogenous Cu ions inhibit cell growth of the metallothionein mutant, which was significantly rescue when supplementing with the ROS scavenger, N-acetylcysteine. To future characterize the molecular mechanism of C. neoformans response to Cu toxicity, we employ iTRAQ with LC-MS/MS analysis, in the presence of exogenous Cu or NAC. Our data showed that an increased Cu level repressed expression of factors involved in protein translation but activates expression of important players in ubiquitin-degradation process. We proposed that the downregulation of protein synthesis and the upregulation of protein degradation were the main strategy of Cu toxicity. The metallothionein mutant showed a higher ubiquitination level under Cu treatment. In addition, MG132 could partially restore the Cu toxicity effect on metallothionein mutant. These results shed new light on the Cu antifungal mechanisms from proteomic profile.
HostingRepository	iProX
AnnounceDate	2021-02-09
AnnouncementXML	Submission_2023-02-20_22:55:03.871.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tianshu Sun
SpeciesList	scientific name: Cryptococcus neoformans var. grubii H99; NCBI TaxID: 235443;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive; Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-09 19:19:11	ID requested
1	2021-02-09 19:19:50	announced
⏵ 2	2023-02-20 22:55:04	announced	2023-02-21: Update information.

Sun T, Li Y, Li Y, Li H, Gong Y, Wu J, Ning Y, Ding C, Xu Y, . Front Cell Infect Microbiol, 11():662404(2021) [pubmed]

submitter keyword: Cryptococcus neoformans, LC-MS/MS

Tianshu Sun
contact affiliation	Medical Research Center, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China
contact email	sun_tianshu@163.com
lab head
Tianshu Sun
contact affiliation	Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences
contact email	sun_tianshu@163.com
dataset submitter

iProX dataset URI