PXD024085 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of Streptomyces coelicolor vesicles |
Description | Streptomyces coelicolor is a model organism for the study of Streptomyces, a genus of Gram-positive bacteria that undergoes a complex life cycle and produces an enormous repertoire of bioactive metabolites and extracellular enzymes. This study investigated the production and characterization of membrane vesicles (MVs) in liquid cultures of S. coelicolor M145 from a structural and biochemical point of view using microscopic, physical and -omics analyzes. Two main populations of MVs, F3 and F4 MVs, with different size and cargo were isolated and purified. S. coelicolor MV cargo is complex and contains many “messages” such as proteins and metabolites. A total of 166 proteins involved in cell metabolism and differentiation, molecular processing and transport was identified in MVs. In particular, a subset of these proteins was protected from the degradation after proteinase K treatment, indicating their localization inside the vesicles. Vesicle proteome includes many stress response proteins which also play an important role in S. coelicolor morpho- physiological differentiation. Moreover, MVs packaged with an array of metabolites such as antibiotics, vitamins, amino acids and components of carbon metabolism. The analysis of S. coelicolor MV cargo will provide informations to elucidate their biogenesis and functions |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-02 |
AnnouncementXML | Submission_2021-11-02_07:27:23.598.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Giovanni Renzone |
SpeciesList | scientific name: Streptomyces coelicolor A3(2); NCBI TaxID: 100226; |
ModificationList | 2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-02-09 07:50:14 | ID requested | |
⏵ 1 | 2021-11-02 07:27:23 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Streptomyces coelicolor vesicles, proteomics, LC-MSMS analysis |
Contact List
Anna Maria Puglia |
contact affiliation | Laboratory of Molecular Microbiology and Biotechnology, STEBICEF Department, University of Palermo, 90128 Palermo, Italy |
contact email | a.maria.puglia@unipa.it |
lab head | |
Giovanni Renzone |
contact affiliation | ISPAAM-CNR |
contact email | giovanni.renzone@cnr.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD024085
- Label: PRIDE project
- Name: Proteomic analysis of Streptomyces coelicolor vesicles