PXD024067-2

PXD024067 is an original dataset announced via ProteomeXchange.

Title	Long non-coding RNA Neat1 is a key of translational regulator in hypoxia
Description	Internal ribosome entry sites (IRESs) drive translation initiation during stress. In response to hypoxia, (lymph)angiogenic factors responsible for tissue revascularization in ischemic diseases are induced by the IRES-dependent mechanism. Here we searched for IRES trans-acting factors (ITAFs) active in early hypoxia in mouse cardiomyocytes. Using knock-down and proteomics approaches, we show a link between a stressed-induced nuclear body, the paraspeckle, and IRES-dependent translation. Our data reveal that the long non-coding RNA Neat1, an essential paraspeckle component, is a key translational regulator, active on IRESs of (lymph)angiogenic and cardioprotective factor mRNAs. In addition, paraspeckle proteins p54nrb and PSPC1 as well as nucleolin and Rps2, two p54nrb-interacting proteins identified by mass spectrometry, are ITAFs for IRES subgroups. Paraspeckle thus appears as the site of IRESome assembly in the nucleus. Polysome PCR array showed that the Neat1_2 isoform widely affects translation of mRNAs containing IRESs, involved in stress response, angiogenesis or cardioprotection.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:00:32.971.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carine Froment
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-08 09:05:57	ID requested
1	2023-01-03 07:34:53	announced
⏵ 2	2023-11-14 08:00:33	announced	2023-11-14: Updated project metadata.

Godet AC, Roussel E, David F, Hantelys F, Morfoisse F, Alves J, Pujol F, Ader I, Bertrand E, Burlet-Schiltz O, Froment C, Henras AK, Vitali P, Lacazette E, Tatin F, Garmy-Susini B, Prats AC, and paraspeckle components are translational regulators in hypoxia. Elife, 11():(2022) [pubmed]

submitter keyword: nanoLC-MS/MS, translational control, hypoxia, cardiomyocyte, Label-free quantitative proteomics

Anne-Catherine Prats
contact affiliation	UMR 1048-I2MC, Inserm, Université de Toulouse, UPS, Toulouse, France
contact email	Anne-Catherine.Prats@inserm.fr
lab head
Carine Froment
contact affiliation	IPBS-CNRS
contact email	carine.froment@ipbs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024067
     1. Label: PRIDE project
     2. Name: Long non-coding RNA Neat1 is a key of translational regulator in hypoxia