PXD024051-1

PXD024051 is an original dataset announced via ProteomeXchange.

Title	ELOF1 is a transcription-coupled DNA repair factor that directs RNA polymerase II ubiquitylation
Description	Cells employ transcription-coupled repair (TCR) to eliminate transcription-blocking DNA lesions. DNA damage-induced binding of the TCR-specific repair factor CSB to RNA polymerase II (RNAPII) triggers RNAPII ubiquitylation at a single lysine (K1268) by the CRL4CSA ubiquitin ligase. How CRL4CSA is specifically directed toward the K1268 site is unknown. Here, we identify ELOF1 as the missing link that facilitates RNAPII ubiquitylation, a key signal for the assembly of downstream repair factors. This function requires its constitutive interaction with RNAPII close to the K1268 site, revealing ELOF1 as a specificity factor that interacts with and positions CRL4CSA for optimal RNAPII ubiquitylation. Drug-genetic interaction screening also reveals a CSB-independent compensatory pathway in which ELOF1 protects cells against DNA replication stress by preventing DNA damage-induced R-loops. Our study offers key insights into the molecular mechanisms of TCR and provides a genetic framework of the interplay between the transcriptional stress response and DNA replication.
HostingRepository	PRIDE
AnnounceDate	2021-04-21
AnnouncementXML	Submission_2021-04-20_22:38:15.292.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Román González-Prieto
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-08 07:11:18	ID requested
⏵ 1	2021-04-20 22:38:15	announced

Dataset with its publication pending

submitter keyword: TC-NER, RNA Polymerase II, transcription, DNA repair.

Román González-Prieto
contact affiliation	Cell and Chemical Biology Leiden University Medical Center The Netherlands
contact email	R.Gonzalez_Prieto@lumc.nl
lab head
Román González-Prieto
contact affiliation	Leiden University Medical Center (LUMC)
contact email	R.Gonzalez_Prieto@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD024051

PRIDE project URI

• PRIDE
  1. PXD024051
     1. Label: PRIDE project
     2. Name: ELOF1 is a transcription-coupled DNA repair factor that directs RNA polymerase II ubiquitylation