PXD024022-2

PXD024022 is an original dataset announced via ProteomeXchange.

Title	High mannose N-glycans on red blood cells as phagocytic ligands, mediating both sickle cell anaemia and resistance to malaria
Description	In both sickle cell disease and malaria, red blood cells (RBCs) are phagocytosed in the spleen, but receptor-ligand pairs mediating uptake have not been identified. Here, we report that patches of high mannose N-glycans, expressed on diseased or oxidized RBC surfaces, bind the mannose receptor (CD206) on phagocytes to mediate clearance. We found that extravascular haemolysis in sickle cell disease correlates with high mannose glycan levels on RBCs. Furthermore, Plasmodium falciparum-infected RBCs expose surface mannose N-glycans, which occur at significantly higher levels on infected RBCs from sickle cell trait subjects compared to those lacking haemoglobin S. This proteomics analysis is to prove the identify of high molecular weight complexes and protease-resistant, lower molecular weight fragments containing spectrin.
HostingRepository	PRIDE
AnnounceDate	2021-03-17
AnnouncementXML	Submission_2021-04-06_03:01:32.004.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aristotelis Antonopoulos
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetic acid derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-05 06:06:27	ID requested
1	2021-03-16 23:27:47	announced
⏵ 2	2021-04-06 03:01:32	announced	2021-04-06: Updated publication reference for PubMed record(s): 33741926.
3	2024-10-22 05:20:07	announced	2024-10-22: Updated project metadata.

Cao H, Antonopoulos A, Henderson S, Wassall H, Brewin J, Masson A, Shepherd J, Konieczny G, Patel B, Williams ML, Davie A, Forrester MA, Hall L, Minter B, Tampakis D, Moss M, Lennon C, Pickford W, Erwig L, Robertson B, Dell A, Brown GD, Wilson HM, Rees DC, Haslam SM, Alexandra Rowe J, Barker RN, Vickers MA, Red blood cell mannoses as phagocytic ligands mediating both sickle cell anaemia and malaria resistance. Nat Commun, 12(1):1792(2021) [pubmed]

submitter keyword: red blood cells, Sickle cells, Malaria

Anne Dell
contact affiliation	Imperial College London, Department of Life Sciences, UK
contact email	a.dell@imperial.ac.uk
lab head
Aristotelis Antonopoulos
contact affiliation	Imperial College London South Kensington Campus Department of Life Sciences
contact email	a.antonopoulos@imperial.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024022
     1. Label: PRIDE project
     2. Name: High mannose N-glycans on red blood cells as phagocytic ligands, mediating both sickle cell anaemia and resistance to malaria