PXD024005-2

PXD024005 is an original dataset announced via ProteomeXchange.

Title	Proteome Differences between Daptomycin-Resistant and WT MRSA Biofilms after Elasnin Treatment
Description	A recent study reported that daptomycin-resistant MRSA (DAPR) strain biofilm is more resistant to daptomycin and vancomycin, as compared to the WT strain biofilm. This pose a great danger since DAPR MRSA is prevalent in clinics and they often form biofilms in medical devices. In this study, we investigate the anti-biofilm activity of elasnin against DAPR MRSA biofilms, and we observed that elasnin is not only effective to eradicate the biofilm of the DAPR strain, but it shows a superior activity compared to the susceptible WT strain. Using proteomics, we compared the proteome profile of the DAPR and the WT strain biofilm cells under elasnin treatment to reveal why elasin is superior against the DAPR strain. Besides, we also employed adaptive laboratory evolution (ALE) experiment by repetitively treating MRSA culture with high dose of elasnin, and generated evolved MRSA strain with increased elasnin tolerance. Using quantitative proteomics, we compared the proteome differences in the elasnin-susceptible WT MRSA and elasnin-tolerant evolved strain.
HostingRepository	PRIDE
AnnounceDate	2021-11-11
AnnouncementXML	Submission_2022-03-02_02:56:15.491.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jordy Evan Sulaiman
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-04 23:14:55	ID requested
1	2021-11-11 01:05:52	announced
⏵ 2	2022-03-02 02:56:16	announced	2022-03-02: Updated publication reference for PubMed record(s): 35196789.
3	2022-03-02 02:56:31	announced	2022-03-02: Updated publication reference for PubMed record(s): 35196789. 2022-03-02: Updated publication reference for PubMed record(s): 35076266, 35196789.

Sulaiman JE, Long L, Qian PY, Lam H, Elasnin Effectively Eradicates Daptomycin-Resistant Methicillin-Resistant Staphylococcus aureus Biofilms. Microbiol Spectr, 10(1):e0232021(2022) [pubmed]

submitter keyword: MRSA, elasnin, daptomycin-resistant, biofilms

Henry Lam
contact affiliation	Chemical and Biological Engineering, The Hong Kong University of Science and Technology (HKUST)
contact email	kehlam@ust.hk
lab head
Jordy Evan Sulaiman
contact affiliation	The Hong Kong University of Science and Technology (HKUST)
contact email	jesulaiman@connect.ust.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD024005

PRIDE project URI

• PRIDE
  1. PXD024005
     1. Label: PRIDE project
     2. Name: Proteome Differences between Daptomycin-Resistant and WT MRSA Biofilms after Elasnin Treatment