PXD023994-1

PXD023994 is an original dataset announced via ProteomeXchange.

Title	Precisely measured protein lifetimes in the brain of aged mice
Description	Aging, which is associated with protein homeostasis defects, is one of the most prominent risk factors for neurodegenerative disorders and the regulation of protein homeostasis is essential for brain health. While several studies have addressed changes in protein abundance due to aging, little is known about differences in protein stability in the mammalian brain proteome. In particular, pre-symptomatic alterations of protein stability might be instrumental for understanding the pathological alterations that occur during neurodegeneration. To explore these alterations, we measured proteome turnover with a pulse-SILAC approach in aged mice from total cortex and cerebellum homogenates and from the respective synaptic-enriched fraction. We compared the stability of young and aged proteomes. Our analyses revealed several differences and the large dataset that we created will serve as a resource for the community to develop approaches that explore these alterations with diagnostic and therapeutic purposes.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_06:44:03.537.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Verena Klüver
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	6x(13)C labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-04 07:24:46	ID requested
⏵ 1	2022-06-09 06:44:04	announced

Kluever V, Russo B, Mandad S, Kumar NH, Alevra M, Ori A, Rizzoli SO, Urlaub H, Schneider A, Fornasiero EF, Protein lifetimes in aged brains reveal a proteostatic adaptation linking physiological aging to neurodegeneration. Sci Adv, 8(20):eabn4437(2022) [pubmed]

submitter keyword: Protein lifetimes, metabolic labeling, brain turnover, synapse, cortex, cerebellum

Eugenio F. Fornasiero
contact affiliation	Department of Neuro- and Sensory Physiology University Medical Center Göttingen Humboldtallee 23 37073 Göttingen
contact email	efornas@gwdg.de
lab head
Verena Klüver
contact affiliation	UMG Neuro- and Sensory Physiology
contact email	verena.klver@stud.uni-goettingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD023994

PRIDE project URI

• PRIDE
  1. PXD023994
     1. Label: PRIDE project
     2. Name: Precisely measured protein lifetimes in the brain of aged mice