PXD023989-2

PXD023989 is an original dataset announced via ProteomeXchange.

Title	Phase separation and stress granule association of SARS-CoV-2 Nucleocapsid protein
Description	The current work identified the protein interactors of SARS-CoV-2 Nucleocapsid RNA binding protein (NCAP or N protein) in lung cancer A-549 cells, using a co-immunoprecipitation strategy, which is coupled with SILAC-based mass spec. A significant proportion of the identified NCAP interacting proteins included stress granule (SG) and immunoregulators. SG nucleator G3BP1 showed specific interaction wit NCAP in unstressed and oxidative- or heat-stressed cells. Following stress treatment, NCAP showed enhanced association with SGs. Recombinant NCAP exhibited in vitro liquid-liquid phase separation (LLPS)to form liquid droplets. RNA stimulated NCAP to develop droplets in vitro and SG assembly in cells, and RNase A treatment completely blocked both of these functions. An RNA intercalating mitoxantrone disrupted NCAP assembly in SGs invitro and in cells. This study provides insight into the biological processes and biophysical properties of the SARS-CoV-2 NCAP and identifies a candidate way to target this protein.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:30:27.660.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Syam Prakash Somasekharan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-04 01:22:02	ID requested
1	2021-11-25 07:43:39	announced
⏵ 2	2024-10-22 05:30:28	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

ProteomeXchange project tag: Covid-19

submitter keyword: SILAC Mass Spec, NCAP, Nucleocapsid,Sars-Cov-2

Dr. Syam Prakash Somasekharan
contact affiliation	Vancouver Prostate Centre, Vancouver, Canada
contact email	syamprakash@gmail.com
lab head
Syam Prakash Somasekharan
contact affiliation	Vancouver Prostate Centre
contact email	syamprakash@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023989
     1. Label: PRIDE project
     2. Name: Phase separation and stress granule association of SARS-CoV-2 Nucleocapsid protein