PXD023971-2

PXD023971 is an original dataset announced via ProteomeXchange.

Title	Biophysical and proteomic analyses suggest functions of Pseudomonas syringae pv tomato DC3000 extracellular vesicles in bacterial growth during plant infection
Description	Vesiculation is a process employed by Gram-negative bacteria to release extracellular vesicles (EVs) into the environment. Bacterial EVs contain molecular cargo from the donor bacterium and play important roles in bacterial survival and growth. Here, we describe EV production in plant-pathogenic Pseudomonas syringae pv. tomato DC3000 (Pto DC3000), the causal agent of bacterial speck disease. Cultured Pto DC3000 exhibited EV structures both on the cell surface and in the vicinity of bacterial cells, observed as outer membrane vesicle (OMV) release. We used in-solution trypsin digestion coupled to mass spectrometry to identify 369 proteins enriched in EVs recovered from cultured Pto DC3000. The predicted localization profile of EV proteins supports the production of EVs also in the form of outer-inner-membrane vesicles (OIMVs). EV production varied slightly between bacterial lifestyles and also occurred in planta. The potential contribution of EVs to Pto DC3000 plant infection was assessed using plant treatments and bioinformatic analysis of the EV-enriched proteins. While these results identify immunogenic activities of the EVs, they also point at roles for EVs in bacterial defences and nutrient acquisition by Pto DC3000.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:06:37.363.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christina Ludwig
SpeciesList	scientific name: Pseudomonas syringae pv. tomato str. DC3000; NCBI TaxID: 223283;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-03 08:27:34	ID requested
1	2023-10-24 10:32:20	announced
⏵ 2	2023-11-14 09:06:38	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:07:57	announced	2024-10-22: Updated project metadata.

Janda M, Rybak K, Krassini L, Meng C, Feitosa-Junior O, Stigliano E, Szulc B, Sklenar J, Menke FLH, Malone JG, Brachmann A, Klingl A, Ludwig C, Robatzek S, DC3000 extracellular vesicles suggest adaptive functions during plant infection. mBio, 14(4):e0358922(2023) [pubmed]

submitter keyword: pattern-triggered immunity, proteomics, Arabidopsis thaliana, NTA, Pto DC3000,Extracellular vesicles, PTI, nanoparticle tracking analysis, EVs

Chen Meng
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry, Freising, TU Munich
contact email	chen.meng@tum.de
lab head
Christina Ludwig
contact affiliation	TU Munich
contact email	tina.ludwig@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023971
     1. Label: PRIDE project
     2. Name: Biophysical and proteomic analyses suggest functions of Pseudomonas syringae pv tomato DC3000 extracellular vesicles in bacterial growth during plant infection