PXD023964-1

PXD023964 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics Analysis of Placenta from Zika Virus Infected Women
Description	Zika Virus (ZIKV) infection has been associated with fetal abnormalities by compromising placental integrity. Flavivirus have shown deregulation of the host proteome, specially extracellular matrix (ECM) proteins. Our hypothesis is that ZIKV infection of placental tissue deregulates specific ECM proteome, allowing a mechanism of persistent infection and loss of placental integrity. Using nine different placental samples from Puerto Rico collected during 2016 ZIKV epidemic, we compared the proteome of five ZIKV infected samples with uninfected controls. Quantitative proteomics was performed using tandem mass tag TMT10plex™ Isobaric Label Reagent Set followed by Q Exactive™ Hybrid Quadrupole Orbitrap Mass Spectrometry. Identification of proteins was performed by Proteome Discover 2.1, and protein fold change in both groups was compared using Limma software. Differentially expressed proteins were analyzed with STRING and Ingenuity Pathway using both the fold change and p-value. TMT analysis showed that ZIKV infected placentas had 94 reviewed differentially abundant proteins, 32 More abundant and 62 Less abundant. STRING analysis results indicate that 45 of the deregulated proteins are cellular components of the ECM and 16 play a role in its structure and organization. Some of the significantly More abundant proteins were Fibrinogen, Vitronectin and Fibronectin. The results we observed in the samples from naturally infected pregnant women may contribute to the understanding of the mechanism (s) employed by ZIKV during placental infection and give insight on novel targets used to disrupt placental function.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_03:59:44.110.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023964
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Abiel Roche
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-03 06:15:14	ID requested
⏵ 1	2022-06-09 03:59:45	announced
2	2024-10-22 05:37:39	announced	2024-10-22: Updated project metadata.

Borges-V, é, lez G, Rosado-Philippi J, Cantres-Rosario YM, Carrasquillo-Carrion K, Roche-Lima A, P, é, rez-Vargas J, Gonz, á, lez-Mart, í, nez A, Correa-Rivas MS, Mel, é, ndez LM, Zika virus infection of the placenta alters extracellular matrix proteome. J Mol Histol, 53(2):199-214(2022) [pubmed]

submitter keyword: Zika Virus (ZIKV), Tandem Mass Tagging (TMT), Congenital Zika Syndrome (CZS)

Dr. Abiel Roche-Lima
contact affiliation	Abiel Roche-Lima, MSc., PhD. Assistant Professor Director, Integrated Informatics Services core IIS-RCMI University of Puerto Rico Medical Science Campus RCMI Program PO Box 365067 San Juan, PR. 00936-5067 Phone: +1 787 758-2525 x 2194
contact email	abiel.roche@upr.edu
lab head
Abiel Roche
contact affiliation	UPR-MSC
contact email	abiel.roche@upr.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023964
     1. Label: PRIDE project
     2. Name: Quantitative Proteomics Analysis of Placenta from Zika Virus Infected Women