PXD023873-1

PXD023873 is an original dataset announced via ProteomeXchange.

Title	AMPylation is a specific lysosomal protein posttranslational modification characteristic for neuronal differentiation
Description	Protein AMPylation is a prevalent posttranslational modification with an emerging role in neurodevelopment and neurodegeneration. Although in metazoans the two highly conserved protein AMP-transferases together with diverse group of AMPylated proteins have been identified using chemical proteomics and biochemical techniques the function of this modification remains largely unknown. Particularly problematic is a localisation of thus far identified AMPylated proteins and putative AMP-transferases. Here, we uncover protein AMPylation as a novel lysosomal protein posttranslational modification characteristic for differentiating neurons. The AMPylated soluble form of exonuclease PLD3 localised in lysosomes shows dramatic increase during the differentiation of neuronal cell lineages. Similar AMPylation pattern has been observed for a lysosomal acid phosphatase ACP2. Our discovery was enabled by combination of chemical proteomics and novel gel-based separation technique of modified and non-modified proteins. Together, our findings expose further the connection between the protein AMPylation and neurodevelopment and reveal a novel lysosomal posttranslational modification.
HostingRepository	PRIDE
AnnounceDate	2021-12-01
AnnouncementXML	Submission_2021-12-01_06:09:36.872.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tobias Becker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-28 09:03:17	ID requested
⏵ 1	2021-12-01 06:09:37	announced
2	2021-12-27 08:31:35	announced	2021-12-27: Updated project metadata.

Dataset with its publication pending

submitter keyword: AMPylation, Lysosome

Dr. Pavel Kielkowski
contact affiliation	LMU Munich, Department of Chemistry
contact email	pavel.kielkowski@cup.lmu.de
lab head
Tobias Becker
contact affiliation	Ludwig-Maximilians-University Junior Group Dr. Kielkowski
contact email	tobias.becker@cup.lmu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023873
     1. Label: PRIDE project
     2. Name: AMPylation is a specific lysosomal protein posttranslational modification characteristic for neuronal differentiation