PXD023854-1

PXD023854 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of Toxoplasma gondii mutants of the mitochondrial and plastidic Iron sulfur cluster synthesis pathways.
Description	Toxoplasma gondii is a parasitic protist that is the agent of toxoplasmosis. It is capable of infecting all mammals, including humans. The infection is mainly asymptomatic in immunocompetent patients, but in case of immunosuppression or for the congenital form of toxoplasmosis it can lead to severe pathologies with a possible fatal outcome. T. gondii contains two organelles of endosymbiotic origin: the mitochondrion and the apicoplast, which is a non-photosynthetic plastid. These organelles contain important biochemical pathways which might be interesting targets for future therapeutic strategies. Iron-sulfur clusters are one of the most ancient and ubiquitous prosthetic groups, and they are required by a variety of proteins involved in important metabolic processes. As for plants, T. gondii has several pathways for biosynthesis of iron-sulfur proteins, located in three different cellular compartments: the cytoplasm, the mitochondrion and the apicoplast. We have investigated the relative contributions of the mitochondrion and the apicoplast to the iron-sulfur proteome of the parasite by generating specific mutants for key proteins of the mitochondrial (TgIscU) and plastidic (TgSufS) pathways, on which we performed a quantitative proteomic analysis.
HostingRepository	PRIDE
AnnounceDate	2021-11-09
AnnouncementXML	Submission_2021-11-09_13:58:30.137.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	sonia hem
SpeciesList	scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-27 10:47:27	ID requested
⏵ 1	2021-11-09 13:58:30	announced

Dataset with its publication pending

submitter keyword: iron sulfur cluster, differentiation, bradyzoite, apicoplast, mitochondrion

Sébastien Besteiro
contact affiliation	LPHI, Univ Montpellier, CNRS, INSERM, Montpellier, France
contact email	sebastien.besteiro@inserm.fr
lab head
sonia hem
contact affiliation	INRA
contact email	sonia.hem@supagro.inra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023854
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of Toxoplasma gondii mutants of the mitochondrial and plastidic Iron sulfur cluster synthesis pathways.