PXD023851-1

PXD023851 is an original dataset announced via ProteomeXchange.

Title	IgE antibodies increase honeybee venom responsiveness and detoxification efficiency of mast cells
Description	In contrast to their clearly defined roles in allergic diseases, the physiologic functions of Immunoglobulin E antibodies (IgEs) and mast cells (MCs) remain enigmatic. Recent research supports the toxin hypothesis, showing that MCs and IgE-related type 2 immune responses can enhance host defense against certain noxious substances, including honeybee venom (BV). However, the mechanisms by which MCs can interfere with BV toxicity are unknown. In this study, we assessed the role of IgE and certain MC products in MC-mediated BV detoxification. We applied in vitro and in vivo fluorescence microscopy imaging, and flow cytometry, fibroblast-based toxicity assays and mass spectrometry to investigate IgE-mediated detoxification of BV cytotoxicity by mouse and human MCs in vitro. Pharmacologic strategies to interfere with MC-derived heparin and proteases helped to define the importance of specific detoxification mechanisms. Venom-specific IgE increased the degranulation and cytokine responses of MCs to BV in vitro. Passive serum sensitization enhanced MC degranulation in vivo. IgE-activated mouse or human MCs exhibited enhanced potential for detoxifying BV by both proteolytic degradation and heparin-related interference with toxicity. Mediators released by IgE-activated human MCs efficiently degraded multiple BV toxins. Our results both reveal that IgE sensitization enhances the MC’s ability to detoxify BV and also assign efficient toxin-neutralizing activity to MC-derived heparin and proteases. Our study thus highlights the potential importance of IgE, MCs, and particular MC products in defense against BV.
HostingRepository	PRIDE
AnnounceDate	2021-11-25
AnnouncementXML	Submission_2021-11-25_06:38:14.202.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023851
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andre Mueller
SpeciesList	scientific name: Apis mellifera (Honeybee); NCBI TaxID: 7460; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-27 10:45:56	ID requested
⏵ 1	2021-11-25 06:38:14	announced

Dataset with its publication pending

submitter keyword: Honeybee venom

host defense

IgE

mast cells

toxin hypothesis

Stephen J. Galli
contact affiliation	Dept. of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, 94305-5176, USA
contact email	sgalli@stanford.edu
lab head
Andre Mueller
contact affiliation	Research Center for Molecular Medicine of the Austrian Academy of Sciences
contact email	amueller@cemm.oeaw.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023851
     1. Label: PRIDE project
     2. Name: IgE antibodies increase honeybee venom responsiveness and detoxification efficiency of mast cells