PXD023840-1

PXD023840 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics attributes aberrant signaling events to a druggable kinome in hepatocellular carcinom
Description	To connect aberrant phospho-signaling events to therapeutic vulnerabilities in hepatocellular carcinoma (HCC), we performed a mass-spectrometry-based phosphoproteomic quantification of human HCC tumors, adjacent tissues and cell lines. We developed an integrated pipeline as the inference of druggable kinome (iDruKin), and computationally inferred differentially activated protein kinases from the phosphoproteomic data, and totally identified 16 kinases as potent drug targets in HCC. Together with experiments in cell lines, tissues and mice, our results not only confirmed MTOR as a major target in HCC, but also predicted the inhibition of CDK4/6 and the activation of PKCs and AMPKs to be promisingly effective in HCC therapy. Additionally, we observed most of these kinases are more significantly dysregulated in recurrent HCCs, and the kinase-activity signature was used to accurately classify HCC samples. Thus, this study demonstrated the potential of iDruKin in identifying drug targets and prognostic markers for HCC and other cancers.
HostingRepository	iProX
AnnounceDate	2023-01-02
AnnouncementXML	Submission_2025-11-17_00:16:38.599.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yao Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-26 22:08:40	ID requested
⏵ 1	2025-11-17 00:16:39	announced

Zhang Z, Zhang Z, Zhang Y, Li Y, Li K, Liu S, Cao J, Li Y, Peng X, Li S, Yin Y, Jiang S, Zuo T, Chang L, Xu Z, Li C, Ding J, Wu J, Zhang W, Wang X, Xia G, Lu S, Xu P, Phosphoproteomics delineates hepatocellular carcinoma subtypes and pinpoints therapeutic targets. Hepatology, 82(6):1432-1449(2025) [pubmed]

submitter keyword: hepatocellular carcinoma, phosphorylation, phosphoproteomics, protein kinase, kinome, MTOR

Ping Xu
contact affiliation	Beijing Proteome Research Center
contact email	xupingghy@gmail.com
lab head
Yao Zhang
contact affiliation	Beijing Proteome Research Center
contact email	zhangyaowsw@163.com
dataset submitter

iProX dataset URI