PXD023822-1

PXD023822 is an original dataset announced via ProteomeXchange.

Title	Impact of sulfamethoxazole on a riverine microbiome
Description	Freshwater environments such as rivers receive effluent discharges from wastewater treatment plants, representing a potential hotspot for antibiotic resistance genes (ARGs). These effluents also contain low levels of different antimicrobials including biocides and antibiotics such as sulfonamides that can be frequently detected in rivers. The impact of such exposure on ARG prevalence and microbial diversity of riverine environment is unknown, so the aim of this study was to investigate the release of a sub-lethal concentration (<4 g L-1) of the sulfonamide compound sulfamethoxazole (SMX) on the river bacterial microbiome using a microflume system. This system was a semi-natural in-vitro microflume using river water (30 L) and sediment, with circulation to mimic river flow. A combination of ‘omics’ approaches were conducted to study the impact of SMX exposure on the microbiomes within the microflumes. Metaproteomics did not show differences in ARGs expression with SMX exposure in water.
HostingRepository	PRIDE
AnnounceDate	2021-07-06
AnnouncementXML	Submission_2021-07-05_21:37:08.824.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023822
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrew Bottrill
SpeciesList	scientific name: wastewater metagenome; NCBI TaxID: 527639;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-26 01:51:23	ID requested
⏵ 1	2021-07-05 21:37:09	announced

Dataset with its publication pending

submitter keyword: Microflumes, Sulfamethoxazole, Antimicrobial Resistance, Metagenomics, Metaproteomics, Warwick-RTP

Professor Elizabeth Wellington
contact affiliation	School of Life Sciences, University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, United Kingdom
contact email	E.M.H.Wellington@warwick.ac.uk
lab head
Andrew Bottrill
contact affiliation	University of Warwick
contact email	a.r.bottrill@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023822
     1. Label: PRIDE project
     2. Name: Impact of sulfamethoxazole on a riverine microbiome