PXD023791-2

PXD023791 is an original dataset announced via ProteomeXchange.

Title	Identification of RON13 binding with with RON4 substrate
Description	Active host cell invasion by the obligate intracellular apicomplexan parasites relies on the formation of a moving junction (MJ), which joins parasite and host cell plasma membranes during entry. Invading Toxoplasma gondii tachyzoites secrete a complex of RON proteins from the rhoptry organelles that provide the components of the MJ on the cytoplasmic side of the host cell membrane. This study highlights the role of a rhoptry resident kinase, RON13, that plays a crucial role during host cell entry and is a virulence factor in the mouse model of infection. Comparative phosphoproteomics demonstrated RON13-dependent phosphorylation of secreted rhoptry proteins and primarily the RONs complex. Structural and biochemical analysis of RON13 revealed the atypical nature of this active secretory pathway kinase, unique to the Apicomplexa.
HostingRepository	PRIDE
AnnounceDate	2021-05-10
AnnouncementXML	Submission_2021-07-20_01:51:12.707.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oscar Vadas
SpeciesList	scientific name: Toxoplasma gondii GT1; NCBI TaxID: 507601;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-25 02:36:40	ID requested
1	2021-05-10 13:42:58	announced
⏵ 2	2021-07-20 01:51:13	announced	2021-07-20: Updated publication reference for PubMed record(s): 34145271.

Lentini G, Ben Chaabene R, Vadas O, Ramakrishnan C, Mukherjee B, Mehta V, Lunghi M, Grossmann J, Maco B, Visentin R, Hehl AB, Korkhov VM, Soldati-Favre D, Structural insights into an atypical secretory pathway kinase crucial for Toxoplasma gondii invasion. Nat Commun, 12(1):3788(2021) [pubmed]

submitter keyword: Toxoplasma gondii, HDX-MS, Kinase, RON13, Secretory pathway

Dominique Soldati-Favre
contact affiliation	University of Geneva, Department of Microbiology and Molecular Medicine
contact email	dominique.soldati-favre@unige.ch
lab head
Oscar Vadas
contact affiliation	University of Geneva, Faculty of Medicine
contact email	oscar.vadas@unige.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD023791

PRIDE project URI

• PRIDE
  1. PXD023791
     1. Label: PRIDE project
     2. Name: Identification of RON13 binding with with RON4 substrate