PXD023780-1

PXD023780 is an original dataset announced via ProteomeXchange.

Title	Multiple unbiased approaches identify oxidosqualene cyclase as the molecular target of a promising anti-leishmanial
Description	Samples were reduced by the addition of TCEP (25 mM final concentration) and incubated at 37 ºC for 10 min. Alkylation was carried out by the addition of iodoacetamide (25 mM final concentration) and incubated at RT for 1 h in the dark. Samples were then digested by the addition of 1:50 LysC (Wako, Japan) in 100 mM triethylammonium bicarbonate (TEAB) followed by incubation at 37 ºC for 6h and then addition of 1:50 trypsin (Thermo) followed by incubation at 37 ºC overnight. The efficiency of sample digestion efficiency was assessed by MS (LTQ XL, Thermo). The samples were then vacuum-dried and resuspended in 100 mM TEAB (100 µL). Samples were then incubated in their respective Tandem Mass Tag™ (TMT) 10-plex reagents (Thermo) for 1 h at RT with agitation. Reactions were quenched by the addition of 5% hydroxylamine for 15 min and each set of samples (treated and vehicle) were pooled in the same tube and dried overnight. The TMTTM-labelled samples were dried and kept at -85 ºC until further analysis.
HostingRepository	PRIDE
AnnounceDate	2021-07-18
AnnouncementXML	Submission_2021-07-18_04:26:20.390.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Victoriano Corpas-Lopez
SpeciesList	scientific name: Leishmania donovani BPK282A1; NCBI TaxID: 981087;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-25 00:25:05	ID requested
⏵ 1	2021-07-18 04:26:21	announced

Paradela LS, Wall RJ, Carvalho S, Chemi G, Corpas-Lopez V, Moynihan E, Bello D, Patterson S, G, ü, ther MLS, Fairlamb AH, Ferguson MAJ, Zuccotto F, Martin J, Gilbert IH, Wyllie S, Multiple unbiased approaches identify oxidosqualene cyclase as the molecular target of a promising anti-leishmanial. Cell Chem Biol, 28(5):711-721.e8(2021) [pubmed]

submitter keyword: leishmania, oxidosqualene synthase, thermal proteome profiling

Susan Wyllie
contact affiliation	Mode of Action group, University of Dundee
contact email	swyllie@dundee.ac.uk
lab head
Victoriano Corpas-Lopez
contact affiliation	University of Dundee
contact email	vcorpaslopez@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD023780

PRIDE project URI

• PRIDE
  1. PXD023780
     1. Label: PRIDE project
     2. Name: Multiple unbiased approaches identify oxidosqualene cyclase as the molecular target of a promising anti-leishmanial