PXD023756-1

PXD023756 is an original dataset announced via ProteomeXchange.

Title	Comparative Proteomic Analysis of Fucosylated Glycoproteins in Bacteroides thetaiotaomicron under Different Polysaccharide Nutrition Conditions
Description	Bacteroides thetaiotaomicron, one of the most eminent representative gut commensal Bacteroides species, is able to use the L-fucose in host-derived and dietary polysaccharides to modify its capsular polysaccharides and glycoproteins through a mammalian-like salvage metabolic pathway. This process is essential for the colonization of the bacteria and for symbiosis with the host. However, despite the importance of fucosylated proteins (FGPs) in Bacteroide thetaiotaomicron, their types, distribution, and functions remain unclear. In this project, the effects of different saccharide (glucose, corn starch, mucin, and fucoidan) nutrition conditions on FGP expressions and fucosylation are investigated using a chemical biological method based on metabolic labeling and bioorthogonal reaction. According to the results of label-free quantification, 559 FGPs (205 downregulated and 354 upregulated) are affected by the dietary conditions. Of these differentially expressed proteins, 65 proteins show extremely sensitive fucosylation levels. Specifically, the fucosylation of the chondroitin sulfate ABC enzyme, Sus proteins, and cationic efflux system proteins varies significantly upon the addition of mucin, corn starch, or fucoidan. Moreover, these polysaccharides can trigger an appreciable increase in the fucosylation level of the two-component system and ammonium transport proteins. These results highlight the efficiency of the combined metabolic glycan labeling and bio-orthogonal reaction in enriching the intestinal Bacteroide glycoproteins. Moreover, it emphasize the sensitivity of Bacteroides fucosylation to polysaccharide nutrition conditions, which allows for the regulation of bacterial growth.
HostingRepository	PRIDE
AnnounceDate	2022-02-08
AnnouncementXML	Submission_2022-02-07_17:50:07.965.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hao Jiang
SpeciesList	scientific name: Bacteroides thetaiotaomicron VPI-5482; NCBI TaxID: 226186;
ModificationList	fucosylated
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-22 06:47:40	ID requested
⏵ 1	2022-02-07 17:50:08	announced

Dataset with its publication pending

submitter keyword: Fucosylated glycoprotein,Click chemistry, Bacteroides thetaiotaomicron, Polysaccharide

Hao Jiang
contact affiliation	Ocean University of China
contact email	haojiang@ouc.edu.cn
lab head
Hao Jiang
contact affiliation	School of Pharrmacy and Medicine, Ocean University of China
contact email	haojiang@ouc.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023756
     1. Label: PRIDE project
     2. Name: Comparative Proteomic Analysis of Fucosylated Glycoproteins in Bacteroides thetaiotaomicron under Different Polysaccharide Nutrition Conditions