PXD023733 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Protein citrullination and homocitrullination profiling in mice using LC-MS/MS |
Description | Citrullination and homocitrullination are two post-translational modifications (PTMs) that may cause protein structural and functional changes. Although they have been linked to various biological processes and disease development, the understanding is still poor because of limitations to enrich, detect and localize them. Herein, we report a biotin thiol tag that enables derivatization, enrichment and confident identification of these two PTMs coupled with mass spectrometry. We apply this method to profile these modifications in different tissues of mice. In total, we identify 1,198 citrullination sites and 108 homocitrullination sites from 619 and 79 proteins, respectively, which is the largest dataset to date. We reveal previously unknown distribution and functions of these two PTMs. We also achieve multiplexing quantitative analysis by combing this method with isotopic labeling strategies. This study contributes to an improved understanding of protein citrullination and homocitrullination, and processes the potential to further decipher their physiological and pathological roles. |
HostingRepository | PRIDE |
AnnounceDate | 2022-12-05 |
AnnouncementXML | Submission_2022-12-04_18:27:36.662.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | ZihuiLi |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | carbamoylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-21 03:53:52 | ID requested | |
⏵ 1 | 2022-12-04 18:27:37 | announced | |
2 | 2023-11-14 08:51:18 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Citrullination,Mouse, Biotin thiol tag, Homocitrullination, LC-MS/MS |
Contact List
LingjunLi |
contact affiliation | Department of Chemistry and School of Pharmacy, University of Wisconsin-Madison, WI, USA |
contact email | lingjun.li@wisc.edu |
lab head | |
ZihuiLi |
contact affiliation | University of Wisconsin Madison |
contact email | zli597@wisc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023733
- Label: PRIDE project
- Name: Protein citrullination and homocitrullination profiling in mice using LC-MS/MS