PXD023705-1

PXD023705 is an original dataset announced via ProteomeXchange.

Title	Tissue extracellular matrix hydrogels as alternative to Matrigel for culturing gastrointestinal organoid
Description	Matrigel, a mouse tumor extracellular matrix (ECM) protein mixture, is an indispensable component of most organoid tissue culture. However, it has limited the utility of organoids for drug development and regenerative medicine due to its tumor-derived origin, batch-to22 batch variation, high cost, and safety issues. Here, we demonstrate that gastrointestinal (GI) tissue-derived ECM hydrogels are a suitable substitute for Matrigel in GI organoid culture. We found that the development and function of GI organoids grown in GI ECM hydrogels are comparable or often superior to those in Matrigel. In addition, GI ECM hydrogels enabled long-term subculture and transplantation of GI organoids by providing GI tissue-mimetic microenvironments. Tissue-specific and age-related ECM profiles of GI ECM hydrogels that affect organoid development were also elucidated through proteomic analysis. Together, our results suggest that ECM hydrogels derived from decellularized GI tissues are an effective alternative to the current gold standard, Matrigel, and produce organoids suitable for GI disease modeling, drug development, and tissue regeneration.
HostingRepository	PRIDE
AnnounceDate	2022-01-31
AnnouncementXML	Submission_2022-01-30_23:20:21.149.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sung-Hyun Jo
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-20 07:25:56	ID requested
⏵ 1	2022-01-30 23:20:21	announced

Dataset with its publication pending

submitter keyword: Tissue extracellular matrix, gastrointestinal organoid

Seung-Woo Cho
contact affiliation	Department of Biotechnology, Stem cell and biomaterial engineering Lab, Yonsei University, Republic of Korea.
contact email	seungwoocho@yonsei.ac.kr
lab head
Sung-Hyun Jo
contact affiliation	Soongsil university
contact email	sh20511@soongsil.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD023705

PRIDE project URI

• PRIDE
  1. PXD023705
     1. Label: PRIDE project
     2. Name: Tissue extracellular matrix hydrogels as alternative to Matrigel for culturing gastrointestinal organoid