PXD023690 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution |
Description | Dynamic change in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for responding and elicit a coordinated response to stimuli. Mass spectrometry (MS)-based proteomics in combination with subcellular fractionation can provide comprehensive maps of spatio-temporal regulation of cells but this is highly challenging involving laborious workflows that do not cover the phosphoproteome level. Here we present a high-throughput workflow based on sequential cell fractionation to profile the global (phospho)proteome dynamics across six distinct subcellular fractions. We benchmarked the workflow by studying spatio-temporal EGFR phospho-signaling dynamics in-vitro in HeLa cells and in-vivo in mouse tissues. Finally, we investigated the spatio-temporal stress signaling, revealing cellular relocation of ribosomal proteins in response to hypertonicity and muscle contraction. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-18 |
AnnouncementXML | Submission_2021-11-18_11:22:16.063.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ana Martinez-Val |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-20 01:03:52 | ID requested | |
⏵ 1 | 2021-11-18 11:22:16 | announced | |
2 | 2021-12-20 11:34:57 | announced | 2021-12-20: Updated publication reference for PubMed record(s): 34876567. |
3 | 2022-02-14 03:40:31 | announced | 2022-02-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Proteomics, phosphoproteomics, spatio-temporal dynamics, DIA |
Contact List
Jesper V. Olsen |
contact affiliation | Group leader, Professor Novo Nordisk Foundation Center for Protein Research University of Copenhagen Faculty of Health and Medical Sciences |
contact email | jesper.olsen@cpr.ku.dk |
lab head | |
Ana Martinez-Val |
contact affiliation | Novo Nordisk Foundation Center for Protein Research |
contact email | ana.mdval@cpr.ku.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD023690 |
PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD023690
- Label: PRIDE project
- Name: Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution