PXD023650 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Robust micro-flow LC-MS/MS for proteome analysis – 38,000 runs and counting |
Description | Micro-flow liquid chromatography tandem mass spectrometry (µLC–MS/MS) is increasingly becoming an alternative to nano-LC–MS/MS for the analysis of proteomes. We have recently demonstrated the potential of a µLC–MS/MS system operating with a 1 mm i.d. × 150 mm column and at a flow rate of 50 µl/min for high-throughput applications. Based on the analysis of ~38,000 samples measured on two instruments over the past two years, we show that the approach is extremely robust. Up to 1,500 analysis were performed within one month and >14,000 samples could be analyzed on a single column without loss of chromatographic performance. Samples included proteomes of cell lines, animal tissue and human body fluids, which were analyzed either with or without prior peptide fractionation or stable isotope labeling. We show that the µLC–MS/MS system is capable of measuring ~2,700 proteins from human plasma and ~5,200 proteins from human urine within one day, demonstrating its potential for in-depth as well as high-throughput clinical application. |
HostingRepository | PRIDE |
AnnounceDate | 2021-02-22 |
AnnouncementXML | Submission_2021-02-21_23:22:11.831.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yangyang Bian |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-18 00:09:23 | ID requested | |
⏵ 1 | 2021-02-21 23:22:12 | announced | |
Publication List
Bian Y, Bayer FP, Chang YC, Meng C, Hoefer S, Deng N, Zheng R, Boychenko O, Kuster B, 000 Runs and Counting. Anal Chem, 93(8):3686-3690(2021) [pubmed] |
Keyword List
submitter keyword: Micro-flow LC, Proteomics, Urine, Plasma, 1.0 mm ID column |
Contact List
Bernhard Kuster |
contact affiliation | Chair of Proteomics and Bioanalytics, Technische Universitaet Muenchen |
contact email | kuster@tum.de |
lab head | |
Yangyang Bian |
contact affiliation | Chair of Proteomics and Bioanalytics, Technical University of Munich |
contact email | yangyang.bian@tum.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD023650 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023650
- Label: PRIDE project
- Name: Robust micro-flow LC-MS/MS for proteome analysis – 38,000 runs and counting