PXD023644-2

PXD023644 is an original dataset announced via ProteomeXchange.

Title	Understanding the role of RbgA in the assembly process of the 50S subunit.
Description	Ribosomes must be formed quickly (to support cellular growth demands) and accurately (to ensure proper translation of the genetic code). The assembly of ribosomes in vivo requires not only the constituent rRNAs and ribosomal proteins (r-proteins), but also assembly factors. Several of these factors are GTPases. However, the molecular role that these GTPases play is not understood. Hypotheses range from serving a regulatory/checkpoint role to serving a direct mechanical role in structural rearrangements (such as serving as an RNA chaperone). The objective of our research is to identify the precise function of one of these essential GTPases in the assembly of the 50S subunit and provide a clear picture for the molecular rearrangements associated with its function. This essential GTPase from Bacillus subtilis, the model organism for Gram-positive bacteria, is RbgA, which constitute a key player in the late maturation steps of the 50S ribosome subunit.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:37:18.890.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marlene Oeffinger
SpeciesList	scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-18 00:06:44	ID requested
1	2022-05-31 02:33:12	announced
⏵ 2	2024-10-22 05:37:19	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Ribosome assembly

50S subunit

uL6, RbgA

YlqF

cryo-electron microscopy.

Marlene Oeffinger
contact affiliation	1-Department of Biochemistry and Molecular Medicine; Université de Montréal; C.P. 6128, Succ. Centre-Ville, Montréal, Québec, H3C 3J7; Canada. 2-Institut de Recherches Cliniques de Montréal, 110 Avenue des Pins Ouest, Montréal, Québec, H2W 1R7; Canada. 3-Faculty of Medicine, Division of Experimental Medicine, McGill University, Montréal, Québec, H3A 1A3; Canada.
contact email	marlene.oeffinger@ircm.qc.ca
lab head
Marlene Oeffinger
contact affiliation	Institut de recherches cliniques Montreal (IRCM) Universite de Montreal
contact email	marlene.oeffinger@ircm.qc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD023644

PRIDE project URI

• PRIDE
  1. PXD023644
     1. Label: PRIDE project
     2. Name: Understanding the role of RbgA in the assembly process of the 50S subunit.