PXD023613 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Minute-scale proteomics with Scanning SWATH and high-flow chromatography |
Description | Great advances have been made to quantify proteomes at depth and high precision. However, the inherent complexity of the proteome still creates huge challenges to deal with large sample series and longitudinal experiments. Here we report a data-independent acquisition (DIA) method, Scanning SWATH, and new software algorithms, that combine the advantages of data-dependent and DIA acquisition. Exploiting a continuous movement of the precursor isolation window to assign precursor mass to the MS2 fragment traces, Scanning SWATH increases the precursor identifications of up to 70% compared to conventional DIA methods on 1-5 minute chromatographic gradients. Moreover, we achieve significant acceleration of the mass spectrometric duty cycles, facilitating the generation of quantitative precise proteomes with high-flow (800 µL/min) chromatography and gradients that can be as fast as 30 seconds. We demonstrate the application of ultra-fast proteomics in drug mode-of-action screening. Scanning SWATH proteomes capture the mode-of-action of azoles and statins acting as fungistatic drugs. |
HostingRepository | PRIDE |
AnnounceDate | 2021-03-02 |
AnnouncementXML | Submission_2021-03-02_07:54:20.948.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christoph Messner |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | iodoacetamide derivatized residue |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-15 06:24:23 | ID requested | |
⏵ 1 | 2021-03-02 07:54:21 | announced | |
2 | 2021-03-29 02:53:27 | announced | 2021-03-29: Updated publication reference for PubMed record(s): 33767396. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: High-throughput, Scanning SWATH, data-independent acquisition, high-flow chromatography, drug screen |
Contact List
Markus Ralser |
contact affiliation | Charité Universitätsmedizin Berlin, Department of Biochemistry, Berlin, Germany |
contact email | markus.ralser@charite.de |
lab head | |
Christoph Messner |
contact affiliation | Francis Crick Institute |
contact email | christoph.messner@crick.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023613
- Label: PRIDE project
- Name: Minute-scale proteomics with Scanning SWATH and high-flow chromatography