PXD023588-1

PXD023588 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics analysis of Elp3 deficient bone marrow derived macrophages aggragates with or without IL-4 treatment
Description	Alternative macrophages are critical in parasites defense, the resolution of inflammation and wound healing. RNA modifications, which are defined as post-transcriptional changes in the chemical composition of RNA molecules, are involved in the immune response.Among tRNA-modifying enzymes which target the tRNA anticodon, Elongator and Cytosolic thiouridylase (Ctu)-1/2 complexes exclusively modify the wobble uridine (U34) base in cytosolic tRNAs. However, the full picture of how mRNA translational factors maintain protein synthesis accuracy and co-translational protein folding are far from being fully understood.To address this question,we evaluated the role of Elp3 on protein in the IL4 induced alternative bone marrow derived macrophages (BMDMs). The BMDMs from widetype and Elp3 myeloid specific knockout mice were treated by IL-4 for 24h or not. Then the aggregates were isolated from these BMDMs and performed by MS.
HostingRepository	PRIDE
AnnounceDate	2022-09-12
AnnouncementXML	Submission_2022-09-12_10:25:06.409.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023588
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dawei Chen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-14 03:12:43	ID requested
⏵ 1	2022-09-12 10:25:06	announced
2	2023-11-14 08:51:58	announced	2023-11-14: Updated project metadata.

10.15252/EMBJ.2021109353;

submitter keyword: mouse bone marrow derived macrophages, aggregates

Alain Chariot
contact affiliation	Laboratory of Medical Chemistry, Interdisciplinary Cluster for Applied Genoproteomics (GIGA) Stem Cells
contact email	alainchariot@uliege.be
lab head
Dawei Chen
contact affiliation	University of Liège
contact email	dawei.chen@uliege.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD023588

PRIDE project URI

• PRIDE
  1. PXD023588
     1. Label: PRIDE project
     2. Name: Quantitative proteomics analysis of Elp3 deficient bone marrow derived macrophages aggragates with or without IL-4 treatment