PXD023551-1

PXD023551 is an original dataset announced via ProteomeXchange.

Title	LFQ proteomics of mouse GABAergic interneurons during traumatic brain injury
Description	Unilateral Traumatic Brain Injury (TBI) causes functional disturbances of the neuronal networks spreading even to the undamaged cortical hemisphere. The phenomenon, referred to as transhemispheric diaschisis, is suggested to be mediated by an imbalance of the strength of glutamatergic, excitatory vs. GABAergic, inhibitory neurotransmission. Here we present evidence that a switch in expression of α Subunits of pore-forming L-Type voltage-gated calcium channels (VGCC), by an expression of CaV1.3 and simultaneous ablation of CaV1.2 in GABAergic interneurons could balance early cortical disturbances manifested as contralateral hyperexcitability in the early phase after TBI. The switch of the VGCC alpha Subunits in GABAergic interneurons was detected using the GAD67-GFP (Glutamate Decarboxylase 67 – Green Fluorescent Protein) Knock-in mouse line. Mice received a TBI with a Controlled Cortical Impact (CCI) to the primary motor and somatosensory cortex at postnatal day 19-21 under anesthesia in vivo. Single GFP+ interneurons located in the undamaged, contralateral cortex were isolated by Fluorescence-Activated Cell Sorting (FACS) and further analyzed by Mass Spectrometry (MS). The switch was associated with an increased excitability of Somatostatin (SST) interneurons and extracellular network activity in acute brain slices in Microelectrode Array (MEA) recordings, which could be restored in presence of isradipine (100 nM), which selectively blocks CaV1.3-containing VGCCs. These data suggest that a switch in alpha.subunits of VGCCs expressed on SST-positive interneurons stabilizes early hyperactivity of the contralateral cortical network at 72 h after TBI, thereby promoting an adaptive mechanism to counterbalance post-traumatic hyperexcitabilty that might lead to epileptogenesis.
HostingRepository	PRIDE
AnnounceDate	2022-01-03
AnnouncementXML	Submission_2022-01-03_12:09:05.063.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Malte Sielaff
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-12 07:20:42	ID requested
⏵ 1	2022-01-03 12:09:06	announced

Ihbe N, Le Prieult F, Wang Q, Distler U, Sielaff M, Tenzer S, Thal SC, Mittmann T, Subunits in L-Type Voltage-Gated Calcium Channels. Cereb Cortex, 32(5):1093-1109(2022) [pubmed]

submitter keyword: Traumatic brain injury, Neuroplasticity, Inhibitory interneurons, L-type voltage-gated calcium channel

Stefan Tenzer
contact affiliation	Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Germany
contact email	tenzer@uni-mainz.de
lab head
Malte Sielaff
contact affiliation	University Medical Center Mainz
contact email	malte.sielaff@uni-mainz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023551
     1. Label: PRIDE project
     2. Name: LFQ proteomics of mouse GABAergic interneurons during traumatic brain injury