PXD023547 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Defining Essential Regulators of Naïve Human Pluripotent Cell Reprogramming |
Description | Defining how human pluripotent cell identity is controlled and in particular how naïve pluripotency is acquired during cell reprogramming is crucial for the future applications of pluripotent stem cells. However, the regulatory pathways of naïve cell reprogramming remain incompletely understood. Here, we used genome-wide CRISPR-Cas9 screening to identify novel regulators of primed to naïve pluripotent stem cell reprogramming, including genes that are essential for reprogramming and genes that normally impede reprogramming and whose targeted deletion led to enhanced reprogramming. Integrated analysis defined specific chromatin complexes and signalling pathways as critical regulators of naïve reprogramming, and were largely distinct from regulators of somatic cell reprogramming. Mechanistically, PRC1.3 and PRDM14 are jointly required to transcriptionally repress developmental and gene regulatory factors to ensure naïve cell reprogramming. Additionally, small molecule inhibitors of reprogramming impediments increased the efficiency of naïve cell reprogramming, and are of practical benefit that can improve on current reprogramming methods. Taken together, we have identified novel regulators controlling the establishment of naïve pluripotency in human cells, which will open up new ways to exploit the full potential of pluripotent stem cells. These results also provide new insights into mechanisms that destabilise and reconfigure cell identity during cell state transitions. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:51:56.831.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Valar Nila Roamio Franklin |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | methylthiolated residue; monohydroxylated residue; deamidated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-12 06:25:07 | ID requested | |
1 | 2022-04-06 02:16:53 | announced | |
⏵ 2 | 2023-11-14 08:51:57 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Pluripotent stem cells |
embryonic stem cells |
pluripotency |
reprogramming |
cell state |
CRISPR |
Polycomb |
PRC1 |
Histone deacetylases |
HDACs. |
Contact List
Clive D' Santos |
contact affiliation | Proteomics Lab Head, CRUK, University of cambridge |
contact email | clivesdsantos@gmail.com |
lab head | |
Valar Nila Roamio Franklin |
contact affiliation | Research Assistant |
contact email | Valar.Franklin@cruk.cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023547
- Label: PRIDE project
- Name: Defining Essential Regulators of Naïve Human Pluripotent Cell Reprogramming