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PXD023544 is an original dataset announced via ProteomeXchange.

Dataset Summary
Titlemir-1 coordinately regulates lysosomal v-ATPase and biogenesis to affect muscle contractility upon proteotoxic challenge during ageing
DescriptionMuscle function relies on the precise architecture of dynamic contractile elements, which must be fine-tuned to maintain motility throughout life. Muscle is also highly plastic, and remodeled in response to stress, growth, neural and metabolic inputs. The evolutionarily conserved muscle microRNA, mir1, regulates distinct aspects of muscle biology during development, but whether it plays a role during ageing is unknown. Here we investigated the role of C. elegans mir-1 in muscle function in response to proteostatic stress during adulthood. mir-1 deletion results in improved mid-life muscle motility, pharyngeal pumping, and organismal longevity under conditions of polyglutamine repeat proteotoxic challenge. We identified multiple vacuolar ATPase subunits as subject to mir-1 control, and the regulatory subunit vha-13/ATP6VIA as a direct target downregulated via its 3’UTR to mediate mir-1 physiology. mir-1 further regulates nuclear localization of lysosomal biogenesis factor HLH-30/TFEB and lysosomal acidification. Our studies reveal that mir-1 coordinately regulates lysosomal v-ATPase and biogenesis to impact muscle function and health during ageing.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIlian Atanassov
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-01-12 03:15:43ID requested
12021-07-13 21:59:01announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: LFQ, worm
Contact List
Adam Antebi
contact affiliationMax Planck Institute for Biology of Ageing, Joseph Stelzmann Strasse 9b, 50931 Cologne, Germany Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50674 Cologne, Germany
contact emailaantebi@age.mpg.de
lab head
Ilian Atanassov
contact affiliationMax Planck Institute for Biology of Aging
contact emailIlian.Atanassov@age.mpg.de
dataset submitter
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Dataset FTP location
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