PXD023540 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | C4PR_LIV: N-terminomic analysis of SARS-CoV-2-infected Vero E6 cells treated with protease inhibitors (Enriched – Variable Search) |
Description | Many events in viral infection are regulated through post-translational modification. In the case of positive-sense RNA viruses such as SARS-CoV-2 which encodes two viral proteases, proteolytic cleavage of both viral and cellular proteins is crucial for productive viral infection. In this work we study proteolysis in the context of SARS-CoV-2 infection of Vero E6 cells, treated with calpeptin or camostat at 12h post-infection and harvested at 24h post-infection. Our goal was Neo-N-termini and lysine residues were labelled at the protein level with TMTpro reagents prior to tryptic digestion. For unenriched samples, the trypsin-digested material was then fractionated offline, and analysed by LC-MS/MS for the study of total protein abundance over the infection timecourse. For enriched samples, unblocked neo-N-termini generated by tryptic digested were labelled with undecanal and depleted, yielding a sample enriched for blocked neo-N-termini (TMTpro, N-terminal acetylation, and pyroGlu). The enriched samples were similarly fractionated offline and analysed by LC-MS/MS. |
HostingRepository | PRIDE |
AnnounceDate | 2021-07-30 |
AnnouncementXML | Submission_2021-07-29_21:15:03.361.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ed Emmott |
SpeciesList | scientific name: Cercopithecus aethiops (Green monkey) (Grivet); NCBI TaxID: 9534; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-12 03:00:52 | ID requested | |
⏵ 1 | 2021-07-29 21:15:03 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
ProteomeXchange project tag: Covid-19 |
submitter keyword: C4PR_LIV: N-terminomic analysis of SARS-CoV-2-infected Vero E6 cells treated with protease inhibitors (Enriched – Variable Search) |
Contact List
Edward Emmott |
contact affiliation | Centre for Proteome Research, University of Liverpool, Liverpool, UK |
contact email | e.emmott@liverpool.ac.uk |
lab head | |
Ed Emmott |
contact affiliation | Centre for Proteome Research, University of Liverpool |
contact email | e.emmott@liverpool.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD023540 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023540
- Label: PRIDE project
- Name: C4PR_LIV: N-terminomic analysis of SARS-CoV-2-infected Vero E6 cells treated with protease inhibitors (Enriched – Variable Search)