PXD023502-2

PXD023502 is an original dataset announced via ProteomeXchange.

Title	GTPase driven maturation of the human mitoribosomal peptidyl transferase center
Description	We combine genetic perturbation with cryo-electron microscopy (cryo-EM) to establish the mechanism of PTC maturation during human mitoribosome biogenesis. Cryo-EM structures of large mitoribosomal subunit (mtLSU) assembly intermediates purified from GTPBP6-deficient cells reveal that GTPBP5 acts in concert with the methyltransferase domain of the NSUN4-MTERF4 complex to facilitate PTC folding. Addition of recombinant GTPBP6 to these assembly intermediates provides the structural basis for subsequent GTPBP6-mediated late PTC maturation and suggests a sequential order of mtLSU biogenesis. Finally, cryo-EM analysis of 55S mitoribosomes treated with GTPBP6 explains how this protein can adopt a dual role in ribosome biogenesis and recycling. Taken together, these results provide the molecular basis for PTC maturation and establish a hierarchical model for late mitoribosome biogenesis.
HostingRepository	PRIDE
AnnounceDate	2021-05-20
AnnouncementXML	Submission_2021-07-20_00:31:36.649.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Linden
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-11 00:04:55	ID requested
1	2021-05-19 22:33:44	announced
⏵ 2	2021-07-20 00:31:37	announced	2021-07-20: Updated publication reference for PubMed record(s): 34135319.

Hillen HS, Lavdovskaia E, Nadler F, Hanitsch E, Linden A, Bohnsack KE, Urlaub H, Richter-Dennerlein R, Structural basis of GTPase-mediated mitochondrial ribosome biogenesis and recycling. Nat Commun, 12(1):3672(2021) [pubmed]

submitter keyword: Mitochondrial ribosome, Mitoribosome, ribosome assembly, PTC maturation, MTERF4-NSUN4, GTPBP5, GTPBP6, ribosome recycling, Obg-HflX superfamily

Prof. Dr. Henning Urlaub
contact affiliation	Max Planck Institute for Biophysical Chemistry and University Medical Center
contact email	Henning.Urlaub@mpibpc.mpg.de
lab head
Andreas Linden
contact affiliation	Max Planck Institute for Biophysical Chemistry and Clinical Chemistry, University Medical Center Göttingen
contact email	Andreas.Linden@mpibpc.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD023502

PRIDE project URI

• PRIDE
  1. PXD023502
     1. Label: PRIDE project
     2. Name: GTPase driven maturation of the human mitoribosomal peptidyl transferase center