PXD023492-1

PXD023492 is an original dataset announced via ProteomeXchange.

Title	Identification of Native Cross-links in Bacillus subtilis Spore Coat Proteins
Description	The resistance properties of the bacterial spores are partially due to spore surface proteins, ~30% of which are said to form an insoluble protein fraction. Previous research has also identified a group of spore coat proteins affected by spore maturation, which exhibit an increased level of inter protein cross-linking. However, the proteins and the types of cross-links involved, previously proposed based on indirect evidence, have yet to be confirmed experimentally. To obtain more insight into the structural basis the proteinaceous component of the spore coat we attempted to identify coat cross-links and the proteins involved using new peptide fractionation and bioinformatic methods. Young (day 1) and matured (day 5) Bacillus subtilis spores of wild type and transglutaminase mutant strains were digested with formic acid and trypsin, and cross-linked peptides were enriched using strong cation exchange chromatography. The enriched cross-linked peptide fractions were subjected to FT-ICR MS/MS and the high-quality fragmentation data obtained were analysed using two specialized software tools, pLink2 and XiSearch, to identify cross-links. This analysis identified specific disulfide bonds between coat proteins CotE-CotE and CotJA-CotJC, obtained evidence for disulfide bonds in the spore crust proteins CotX, CotY and CotZ, and identified dityrosine and eplison-(gamma)-glutamyl-lysine cross-linked coat proteins. The findings in this report are the first direct biochemical data on protein cross-linking in the spore coat and the first direct evidence for the crosslinked building blocks of the highly ordered and resistant structure called the spore coat.
HostingRepository	MassIVE
AnnounceDate	2021-10-09
AnnouncementXML	Submission_2021-10-09_02:21:01.191.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gertjan Kramer
SpeciesList	scientific name: Bacillus subtilis; NCBI TaxID: 1423;
ModificationList	Oxidation; Deamidated; Carbamidomethyl
Instrument	apex Q

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-09 15:03:26	ID requested
⏵ 1	2021-10-09 02:21:02	announced

Ursem R, Swarge B, Abhyankar WR, Buncherd H, de Koning LJ, Setlow P, Brul S, Kramer G, Spore Coat Proteins. J Proteome Res, 20(3):1809-1816(2021) [pubmed]

submitter keyword: Spore coat, Bacillus subtilis, Disulfide bridges, Protein crosslinks

Stanley Brul
contact affiliation	Swammerdam Institute for Life Sciences, University of Amsterdam
contact email	s.brul@uva.nl
lab head
Gertjan Kramer
contact affiliation	Universiteit van Amsterdam
contact email	gertjan.kramer@uva.nl
dataset submitter

MassIVE dataset URI

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