PXD023448-1

PXD023448 is an original dataset announced via ProteomeXchange.

Title	Esterification Reaction: An Unexpected Side Reaction During Glycopeptide and Glycan Storages in Formic Acid Solution
Description	Glycosylation is one of the most important co- and post-translational modifications on proteins. In glycomics and glycoproteomics studies, the released glycans or intact glycopeptides are analyzed by tandem mass spectrometry to achieve their large-scale analyses. Prior to LC-MS/MS analyses, the glycan and intact glycopeptide samples are normally dissolved in formic acid (FA) solution, and sometimes stored at -20 °C or lower temperatures. In this study, we show that an unexpected +28 Da modification would occur in a time-dependent manner when the glycan and glycopeptide samples were stored in FA solution at -20 °C. Additional evidences suggested that this unexpected modification should be mainly caused by the esterification reaction between the hydroxyl group of glycans and FA. As this modification would reduce the glycopeptide identification and/or increase false positive results, once the glycan and glycopeptide samples have been dissolved in FA solution, it should not be stored at -20 °C.
HostingRepository	PRIDE
AnnounceDate	2025-12-22
AnnouncementXML	Submission_2025-12-21_17:07:54.872.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liuyi Dang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-07 02:06:44	ID requested
⏵ 1	2025-12-21 17:07:55	announced

10.1016/j.mcpro.2025.101085;

Kothlow K, Schramm HM, Markuson KA, Russell JH, Sutherland E, Veth TS, Zhang R, Duboff AG, Tejus VR, McDermott LE, Dr, ä, ger LS, Riley NM, Extracting Informative Glycan-Specific Ions From Glycopeptide MS/MS Spectra With GlyCounter. Mol Cell Proteomics, 24(12):101085(2025) [pubmed]

submitter keyword: Glycoproteomics, Formic acid, Glycomics

Sun Shisheng
contact affiliation	College of Life Science, Northwest University, Xi’an, 710069, P. R. China
contact email	suns@nwu.edu.cn
lab head
Liuyi Dang
contact affiliation	Northwest University, China
contact email	liuyi.dang@outlook.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023448
     1. Label: PRIDE project
     2. Name: Esterification Reaction: An Unexpected Side Reaction During Glycopeptide and Glycan Storages in Formic Acid Solution