PXD023414-1

PXD023414 is an original dataset announced via ProteomeXchange.

Title	Global mapping of RNA-binding proteins in multi-drug resistant Staphylococcus aureus using PTex
Description	Phenol Toluol extraction (PTex) carried out two steps organic-phase separation: first pH neutral phenol–Toluol extraction separated RNA and protein from DNA: RNA and proteins to the top aqueous phase but DNA and membranes in the interface. Aqueous phase then went through two rounds acid-phenol extraction, resulting RNA in the top aqueous phase, proteins bottom organic phase and RNA-protein complexes in the interface. After carefully isolated the interface, ethanol precipitation was carried on for purifying RNA-protein adducts (Urdaneta et al, 2019; Smith et al, 2020)
HostingRepository	PRIDE
AnnounceDate	2022-05-31
AnnouncementXML	Submission_2022-05-31_01:53:22.630.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liangcui Chu
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-05 01:34:16	ID requested
⏵ 1	2022-05-31 01:53:23	announced

Chu LC, Arede P, Li W, Urdaneta EC, Ivanova I, McKellar SW, Wills JC, Fr, ö, hlich T, von Kriegsheim A, Beckmann BM, Granneman S, The RNA-bound proteome of MRSA reveals post-transcriptional roles for helix-turn-helix DNA-binding and Rossmann-fold proteins. Nat Commun, 13(1):2883(2022) [pubmed]

submitter keyword: Staphylococcus aureus, RNA-binding proteins, protein-RNA interactions, UV cross-linking, RBPome, RBDome, organic phase purification

Sander Granneman
contact affiliation	Medical Research Council Senior Research Fellow Associate Professor in RNA Biochemistry and Research Group Leader Centre for Synthetic and Systems Biology (SynthSys) University of Edinburgh Max Born Crescent CH Waddington Building, room 3.06 EH9 3BF Edinburgh UK
contact email	Sander.Granneman@ed.ac.uk
lab head
Liangcui Chu
contact affiliation	Edinburgh University
contact email	chuliangcui@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD023414

PRIDE project URI

• PRIDE
  1. PXD023414
     1. Label: PRIDE project
     2. Name: Global mapping of RNA-binding proteins in multi-drug resistant Staphylococcus aureus using PTex