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PXD023414-1

PXD023414 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGlobal mapping of RNA-binding proteins in multi-drug resistant Staphylococcus aureus using PTex
DescriptionPhenol Toluol extraction (PTex) carried out two steps organic-phase separation: first pH neutral phenol–Toluol extraction separated RNA and protein from DNA: RNA and proteins to the top aqueous phase but DNA and membranes in the interface. Aqueous phase then went through two rounds acid-phenol extraction, resulting RNA in the top aqueous phase, proteins bottom organic phase and RNA-protein complexes in the interface. After carefully isolated the interface, ethanol precipitation was carried on for purifying RNA-protein adducts (Urdaneta et al, 2019; Smith et al, 2020)
HostingRepositoryPRIDE
AnnounceDate2022-05-31
AnnouncementXMLSubmission_2022-05-31_01:53:22.630.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLiangcui Chu
SpeciesList scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-01-05 01:34:16ID requested
12022-05-31 01:53:23announced
Publication List
Chu LC, Arede P, Li W, Urdaneta EC, Ivanova I, McKellar SW, Wills JC, Fr, ö, hlich T, von Kriegsheim A, Beckmann BM, Granneman S, The RNA-bound proteome of MRSA reveals post-transcriptional roles for helix-turn-helix DNA-binding and Rossmann-fold proteins. Nat Commun, 13(1):2883(2022) [pubmed]
Keyword List
submitter keyword: Staphylococcus aureus, RNA-binding proteins, protein-RNA interactions, UV cross-linking, RBPome, RBDome, organic phase purification
Contact List
Sander Granneman
contact affiliationMedical Research Council Senior Research Fellow Associate Professor in RNA Biochemistry and Research Group Leader Centre for Synthetic and Systems Biology (SynthSys) University of Edinburgh Max Born Crescent CH Waddington Building, room 3.06 EH9 3BF Edinburgh UK
contact emailSander.Granneman@ed.ac.uk
lab head
Liangcui Chu
contact affiliationEdinburgh University
contact emailchuliangcui@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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