PXD023414 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Global mapping of RNA-binding proteins in multi-drug resistant Staphylococcus aureus using PTex |
Description | Phenol Toluol extraction (PTex) carried out two steps organic-phase separation: first pH neutral phenol–Toluol extraction separated RNA and protein from DNA: RNA and proteins to the top aqueous phase but DNA and membranes in the interface. Aqueous phase then went through two rounds acid-phenol extraction, resulting RNA in the top aqueous phase, proteins bottom organic phase and RNA-protein complexes in the interface. After carefully isolated the interface, ethanol precipitation was carried on for purifying RNA-protein adducts (Urdaneta et al, 2019; Smith et al, 2020) |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-31 |
AnnouncementXML | Submission_2022-05-31_01:53:22.630.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Liangcui Chu |
SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-05 01:34:16 | ID requested | |
⏵ 1 | 2022-05-31 01:53:23 | announced | |
Publication List
Chu LC, Arede P, Li W, Urdaneta EC, Ivanova I, McKellar SW, Wills JC, Fr, ö, hlich T, von Kriegsheim A, Beckmann BM, Granneman S, The RNA-bound proteome of MRSA reveals post-transcriptional roles for helix-turn-helix DNA-binding and Rossmann-fold proteins. Nat Commun, 13(1):2883(2022) [pubmed] |
Keyword List
submitter keyword: Staphylococcus aureus, RNA-binding proteins, protein-RNA interactions, UV cross-linking, RBPome, RBDome, organic phase purification |
Contact List
Sander Granneman |
contact affiliation | Medical Research Council Senior Research Fellow Associate Professor in RNA Biochemistry and Research Group Leader Centre for Synthetic and Systems Biology (SynthSys) University of Edinburgh Max Born Crescent CH Waddington Building, room 3.06 EH9 3BF Edinburgh UK |
contact email | Sander.Granneman@ed.ac.uk |
lab head | |
Liangcui Chu |
contact affiliation | Edinburgh University |
contact email | chuliangcui@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023414
- Label: PRIDE project
- Name: Global mapping of RNA-binding proteins in multi-drug resistant Staphylococcus aureus using PTex